注册 | 登录
所有产品
  • 所有产品
  • 一抗
  • 裂解液
>   首页   >   产品   >   一抗   >   RPS10 Antibody (C-Term)   

RPS10 Antibody (C-Term)

Purified Rabbit Polyclonal Antibody (Pab)

     
标注"皇冠"的产品,是满足高质量标准的产品。
了解更多...
  • 3 - RPS10 Antibody (C-Term) AP22038b
    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling RPS10 with AP22038b at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-rabbit IgG (NK179883) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on HeLa cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin (PD18466410) at 1/100 dilution (red).The nuclear counter stain is DAPI (blue).
  • 4 - RPS10 Antibody (C-Term) AP22038b
    Overlay histogram showing Hela cells stained with AP22038b (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP22038b, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • 1 - RPS10 Antibody (C-Term) AP22038b
    All lanes : Anti-RPS10 Antibody (C-Term) at 1:2000 dilution Lane 1: A431 whole cell lysate Lane 2: Jurkat whole cell lysate Lane 3: Hela whole cell lysate Lane 4: K562 whole cell lysate Lane 5: C6 whole cell lysate Lane 6: mouse brain lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 19 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 产品详情
  • 实验流程
  • 背景知识
Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IF, FC, WB, E
Primary Accession P46783
Other Accession Q3T0F4
Reactivity Human, Mouse, Rat
Predicted Bovine
Host Rabbit
Clonality polyclonal
Isotype Rabbit Ig
Calculated MW 18898 Da
Additional info
Gene ID 6204
Other Names 40S ribosomal protein S10, RPS10
Target/Specificity This RPS10 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 96-129 amino acids from human RPS10.
Dilution IF~~1:25
FC~~1:25
WB~~1:2000
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsRPS10 Antibody (C-Term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RPS10
Function Component of the 40S ribosomal subunit.
Cellular Location Cytoplasm. Nucleus, nucleolus. Note=Localized in the granular component (GC) region of the nucleolus Methylation is required for its localization in the GC region Colocalizes with NPS1 in the GC region of the nucleolus

BACKGROUND

Component of the 40S ribosomal subunit.

REFERENCES

Frigerio J.-M.,et al.Biochim. Biophys. Acta 1262:64-68(1995).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Mungall A.J.,et al.Nature 425:805-811(2003).
Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
Vladimirov S.N.,et al.Eur. J. Biochem. 239:144-149(1996).

FeedBack

终于等到您。感谢您使用ABGENT(百奇生物)抗体产品。
点击下方“我要评价 ”按钮提交您的反馈信息,您的反馈和评价是我们最宝贵的财富之一,
我们将在1-3个工作日内处理您的反馈信息。

如有疑问,联系:0512-69552906 tech.abgent@wuxiapptec.com.


我要评价