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PHF6 Antibody (C-Term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 3 - PHF6 Antibody (C-Term) AP21978b
    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling PHF6 with AP21978b at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-rabbit IgG (NK179883) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing nucleus staining on HeLa cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin (PD18466410) at 1/100 dilution (red).
  • 1 - PHF6 Antibody (C-Term) AP21978b
    All lanes : Anti-PHF6 Antibody (C-Term) at 1:2000 dilution Lane 1: 293T/17 whole cell lysate Lane 2: A431 whole cell lysate Lane 3: Hela whole cell lysate Lane 4: Jurkat whole cell lysate Lane 5: K562 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 41 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IF, WB, E
Primary Accession Q8IWS0
Other Accession Q08DR0, Q5R5Z2
Reactivity Human
Predicted Bovine
Host Rabbit
Clonality polyclonal
Isotype Rabbit Ig
Calculated MW 41290 Da
Additional info
Gene ID 84295
Other Names PHD finger protein 6, PHD-like zinc finger protein, PHF6, CENP-31, KIAA1823
Target/Specificity This PHF6 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 315-349 amino acids from human PHF6.
Dilution IF~~1:25
WB~~1:2000
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPHF6 Antibody (C-Term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PHF6
Synonyms CENP-31, KIAA1823
Function Transcriptional regulator that associates with ribosomal RNA promoters and suppresses ribosomal RNA (rRNA) transcription.
Cellular Location Nucleus. Nucleus, nucleolus. Chromosome, centromere, kinetochore Note=Nuclear, it particularly localizes to the nucleolus
Tissue Location Ubiquitously expressed.

BACKGROUND

Transcriptional regulator that associates with ribosomal RNA promoters and suppresses ribosomal RNA (rRNA) transcription.

REFERENCES

Lower K.M.,et al.Nat. Genet. 32:661-665(2002).
Nagase T.,et al.DNA Res. 8:85-95(2001).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Ross M.T.,et al.Nature 434:325-337(2005).
Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.

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