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TPRA1 Antibody (N-Term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 4 - TPRA1 Antibody (N-Term) AP21977a
    Overlay histogram showing U-2OS cells stained with AP21977a (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP21977a, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • 1 - TPRA1 Antibody (N-Term) AP21977a
    All lanes : Anti-TPRA1 Antibody (N-Term) at 1:2000 dilution Lane 1: human heart lysate Lane 2: mouse heart lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 41 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
FC, WB, E
Primary Accession Q86W33
Reactivity Human, Mouse
Host Rabbit
Clonality polyclonal
Isotype Rabbit Ig
Calculated MW 41053 Da
Additional info
Gene ID 131601
Other Names Transmembrane protein adipocyte-associated 1, Integral membrane protein GPR175, Transmembrane protein 227, TPRA1, GPR175, TMEM227
Target/Specificity This TPRA1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 16-47 amino acids from human TPRA1.
Dilution FC~~1:25
WB~~1:2000
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsTPRA1 Antibody (N-Term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TPRA1
Synonyms GPR175, TMEM227
Cellular Location Membrane; Multi-pass membrane protein.
Tissue Location Ubiquitous, with higher levels in heart, placenta and kidney.
Research Areas

REFERENCES

Saito T.,et al.Submitted (JAN-2000) to the EMBL/GenBank/DDBJ databases.
Wan D.,et al.Proc. Natl. Acad. Sci. U.S.A. 101:15724-15729(2004).
Muzny D.M.,et al.Nature 440:1194-1198(2006).
Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
Yang H.,et al.Endocrinology 140:2859-2867(1999).

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