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>   首页   >   产品   >   一抗   >   细胞生物学   >   SYVN1 (HRD1) Antibody (N-term)   

SYVN1 (HRD1) Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - SYVN1 (HRD1) Antibody (N-term) AP2184b
    Western blot analysis of anti-HRD1 Pab (Cat. #AP2184b) in mouse brain tissue lysate (35ug/lane). HRD1(arrow) was detected using the purified Pab.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, E
Primary Accession Q86TM6
Other Accession Q5XHH7, Q6NRL6, Q9DBY1, Q8N6E8
Reactivity Human
Predicted Mouse, Xenopus
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Additional info
Gene ID 84447
Other Names E3 ubiquitin-protein ligase synoviolin, 632-, Synovial apoptosis inhibitor 1, SYVN1, HRD1, KIAA1810
Target/Specificity This SYVN1 (HRD1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 58-88 amino acids from the N-terminal region of human SYVN1 (HRD1).
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSYVN1 (HRD1) Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SYVN1
Synonyms HRD1, KIAA1810
Function Acts as an E3 ubiquitin-protein ligase which accepts ubiquitin specifically from endoplasmic reticulum-associated UBC7 E2 ligase and transfers it to substrates, promoting their degradation (PubMed:12459480, PubMed:12646171, PubMed:12975321, PubMed:14593114, PubMed:16289116, PubMed:16847254, PubMed:17059562, PubMed:17141218, PubMed:17170702, PubMed:22607976, PubMed:26471130). Component of the endoplasmic reticulum quality control (ERQC) system also called ER-associated degradation (ERAD) involved in ubiquitin-dependent degradation of misfolded endoplasmic reticulum proteins (PubMed:12459480, PubMed:12646171, PubMed:12975321, PubMed:14593114, PubMed:16289116, PubMed:16847254, PubMed:17059562, PubMed:17141218, PubMed:17170702, PubMed:22607976, PubMed:26471130). Also promotes the degradation of normal but naturally short-lived proteins such as SGK. Protects cells from ER stress-induced apoptosis. Protects neurons from apoptosis induced by polyglutamine-expanded huntingtin (HTT) or unfolded GPR37 by promoting their degradation (PubMed:17141218). Sequesters p53/TP53 in the cytoplasm and promotes its degradation, thereby negatively regulating its biological function in transcription, cell cycle regulation and apoptosis (PubMed:17170702). Mediates the ubiquitination and subsequent degradation of cytoplasmic NFE2L1 (By similarity).
Cellular Location Endoplasmic reticulum membrane; Multi-pass membrane protein
Tissue Location Ubiquitously expressed, with highest levels in liver and kidney (at protein level). Up-regulated in synovial tissues from patients with rheumatoid arthritis (at protein level).
Research Areas
Activating transcription factor 6 limits intracellular accumulation of mutant α(1)-antitrypsin Z and mitochondrial damage in hepatoma cells.
Author : Smith SE, Granell S, Salcedo-Sicilia L, Baldini G, Egea G, Teckman JH, Baldini G.
J Biol Chem. 2011 Dec 2;286(48):41563-77. doi: 10.1074/jbc.M111.280073. Epub 2011 Oct 5.
21976666
Dual role of ancient ubiquitous protein 1 (AUP1) in lipid droplet accumulation and endoplasmic reticulum (ER) protein quality control.
Author : Klemm EJ, Spooner E, Ploegh HL.
J Biol Chem. 2011 Oct 28;286(43):37602-14. doi: 10.1074/jbc.M111.284794. Epub 2011 Aug 20.
21857022
Cutting Edge: Selective role of ubiquitin in MHC class I antigen presentation.
Author : Huang L1, Marvin JM, Tatsis N, Eisenlohr LC.
J Immunol. 2011 Feb 15;186(4):1904-8. doi: 10.4049/jimmunol.1003411. Epub 2011 Jan 14.
21239720

BACKGROUND

HRD1 is a ubiquitin ligase whose expression is induced by the unfolded protein response (UPR) following endoplasmic reticulum stress. Expression of HRD1 protects cells from apoptosis by inducing degradation of abnormally processed proteins that accumulate in the endoplasmic reticulum. HRD1 is expressed in many tissues, strongly expressed in brain, pancreas, liver, kidney and skeletal muscle. Synoviolin/Hrd1 (expressed in rheumatoid synovium) is reported to be a novel causative factor for arthropathy by triggering synovial cell outgrowth through its antiapoptotic effects. HRD1 contains one ring-type zinc finger.

REFERENCES

Kikkert, M., et al., J. Biol. Chem. 279(5):3525-3534 (2004). Amano, T., et al., Genes Dev. 17(19):2436-2449 (2003). Nadav, E., et al., Biochem. Biophys. Res. Commun. 303(1):91-97 (2003). Kaneko, M., et al., FEBS Lett. 532 (1-2), 147-152 (2002) (): ().

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