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MICB Antibody (N-Term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - MICB Antibody (N-Term) AP21778a
    All lanes : Anti-MICB Antibody (N-Term) at 1:1000-1:2000 dilution Lane 1: HepG2 whole cell lysate Lane 2: Hela whole cell lysate Lane 3: MCF-7 whole cell lysate Lane 4: Jurkat whole cell lysate Lane 5: Daudi whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 43 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, E
Primary Accession Q29980
Reactivity Human
Host Rabbit
Clonality polyclonal
Isotype Rabbit Ig
Additional info
Gene ID 4277
Other Names MHC class I polypeptide-related sequence B, MIC-B, MICB {ECO:0000312|EMBL:CAA628231}
Target/Specificity This MICB antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 65-98 amino acids from human MICB.
Dilution WB~~1:1000-1:2000
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMICB Antibody (N-Term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MICB {ECO:0000312|EMBL:CAA62823.1}
Function Seems to have no role in antigen presentation. Acts as a stress-induced self-antigen that is recognized by gamma delta T cells. Ligand for the KLRK1/NKG2D receptor. Binding to KLRK1 leads to cell lysis.
Cellular Location Cell membrane {ECO:0000250|UniProtKB:Q29983}; Single-pass type I membrane protein {ECO:0000250|UniProtKB:Q29983}. Note=Binding to human cytomegalovirus glycoprotein UL16 causes sequestration in the endoplasmic reticulum. {ECO:0000250|UniProtKB:Q29983, ECO:0000269|PubMed:12782710}
Tissue Location Widely expressed with the exception of the central nervous system where it is absent. Expressed in many, but not all, epithelial tumors of lung, breast, kidney, ovary, prostate and colon. In hepatocellular carcinomas, expressed in tumor cells but not in surrounding non-cancerous tissue
Research Areas

BACKGROUND

Seems to have no role in antigen presentation. Acts as a stress-induced self-antigen that is recognized by gamma delta T cells. Ligand for the KLRK1/NKG2D receptor. Binding to KLRK1 leads to cell lysis.

REFERENCES

Bahram S.,et al.Immunogenetics 43:230-233(1996).
Bahram S.,et al.Immunogenetics 45:161-162(1996).
Martinez-Borra J.,et al.Hum. Immunol. 68:705-707(2007).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Hirakawa M.,et al.Submitted (DEC-2004) to the EMBL/GenBank/DDBJ databases.

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