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Ubiquilin1 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 3 - Ubiquilin1 Antibody (Center) AP2176c
    Immunofluorescent analysis of Hela cells, using Ubiquilin1 Antibody (Center) (Cat. #AP2176c). AP2176c was diluted at 1:100 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Dylight Fluor® 554 (red) conjugated Phalloidin (red).
  • 1 - Ubiquilin1 Antibody (Center) AP2176c
    Ubiquilin1 Antibody (P311) (Cat. #AP2176c) western blot analysis in SH-SY5Y cell line lysates (35ug/lane).This demonstrates the Ubiquilin1 antibody detected the Ubiquilin1 protein (arrow).
  • 14 - Ubiquilin1 Antibody (Center) AP2176c
    Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, IF, E
Primary Accession Q9UMX0
Other Accession Q9QZM0, Q9UHD9, Q9JJP9, Q8R317, Q8IXS9
Reactivity Human, Mouse
Predicted Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 62519 Da
Additional info
Gene ID 29979
Other Names Ubiquilin-1, Protein linking IAP with cytoskeleton 1, PLIC-1, hPLIC-1, UBQLN1, DA41, PLIC1
Target/Specificity This Ubiquilin1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 296-326 amino acids from the Central region of human Ubiquilin1.
Dilution IF~~1:100
WB~~1:1000
IHC-P~~1:50~100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsUbiquilin1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name UBQLN1
Synonyms DA41, PLIC1
Function Links CD47 to the cytoskeleton. Promotes the surface expression of GABA-A receptors. Promotes the accumulation of uncleaved PSEN1 and PSEN2 by stimulating their biosynthesis. Has no effect on PSEN1 and PSEN2 degradation.
Cellular Location Cytoplasm. Nucleus. Note=Detected in neuronal processes and at synapses (By similarity). Detected in cytosolic puncta that may correspond to autophagosomes.
Tissue Location Ubiquitous. Highly expressed throughout the brain; detected in neurons and in neuropathological lesions, such as neurofibrillary tangles and Lewy bodies. Highly expressed in heart, placenta, pancreas, lung, liver, skeletal muscle and kidney.
Research Areas
APP modulates KCC2 expression and function in hippocampal GABAergic inhibition.
Author : Chen M1,Wang J1,Jiang J2,Zheng X3,Justice NJ4,Wang K1,Li Y1,Ran X5,Huo Q1,Zhang J1,Li H6,Lu N1,Wang Y1,Zheng H6,Long C1,Yang L2.
Elife. 2017 Jan 5;6. pii: e20142. doi: 10.7554/eLife.20142. [Epub ahead of print]
28054918
Tau pathology in frontotemporal lobar degeneration with C9ORF72 hexanucleotide repeat expansion.
Author : Bieniek KF, Murray ME, Rutherford NJ, Castanedes-Casey M, DeJesus-Hernandez M, Liesinger AM, Baker MC, Boylan KB, Rademakers R, Dickson DW.
Acta Neuropathol. 2013 Feb;125(2):289-302. doi: 10.1007/s00401-012-1048-7. Epub 2012 Sep 28.
23053135
UBQLN1 interacts with SPEM1 and participates in spermiogenesis.
Author : Bao J, Zhang J, Zheng H, Xu C, Yan W.
Mol Cell Endocrinol. 2010 Oct 7;327(1-2):89-97. doi: 10.1016/j.mce.2010.06.006. Epub 2010 Jun 14.
20558241

BACKGROUND

Ubiquilin 1 (UBQLN1), also known as DA41, was isolated from an adult rat lung cDNA library, and encodes a cellular protein that associates with DAN.1 DAN expression is reduced in rat fibroblast 3Y1 cells transformed with mouse sarcoma virus and in rodent fibroblasts transformed with a variety of oncogenes. The DAN-DA41 interaction is mediated through the N-terminal domain and a cysteine-knot region of DAN. Human DA41 encodes a 589-amino acid protein with 86% amino acid sequence identity with rat protein.2 DA41 expression is regulated in a cell cycle-dependent manner. PLIC1 and PLIC2 (UBQLN2) are homologs of the mouse Plics (proteins linking integrin-associated protein (IAP) and cytoskeleton) and the yeast Dsk2 protein. PLIC1, also called UBQLN1, shares 72% amino acid identity with PLIC2,3 Two motifs are conserved in the mammalian PLICs and yeast Dsk2, an N-terminal ubiquitin-like (UBL) domain and a C-terminal ubiquitin-associated (UBA) domain. Unlike ubiquitin, the UBL domain of the PLICs does not have a diglycine motif in its C terminus. The UBA domain is present in multiple enzyme classes of the ubiquitination machinery. Human PLICs associate with both proteasomes and ubiquitin ligases in large complexes. Overexpression of PLICs impairs the in vivo degradation of 2 unrelated ubiquitin-dependent proteasome substrates, p53 and I-kappa-B-alpha (NFKBIA), but not a ubiquitin-independent substrate. PLICs may link the ubiquitination machinery to the proteasome to affect in vivo protein degradation. The DA41 gene maps to chromosome 9q21.2-q21.3, a position overlapping a candidate tumor suppressor locus for bladder cancer.2

REFERENCES

Massey, L.K., et al., J. Alzheimers Dis. 6(1):79-92 (2004). Gao, L., et al., J. Virol. 77(7):4149-4159 (2003). Mah, A.L., et al., J. Cell Biol. 151(4):847-862 (2000). Kleijnen, M.F., et al., Mol. Cell 6(2):409-419 (2000). Hanaoka, E., et al., J. Hum. Genet. 45(3):188-191 (2000).

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