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CBX8 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - CBX8 Antibody (Center) AP21434c
    All lanes : Anti-CBX8 Antibody (Center) at 1:8000 dilution Lane 1: Jurkat whole cell lysates Lane 2: rat brain lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 43 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 14 - CBX8 Antibody (Center) AP21434c
    Formalin-fixed and paraffin-embedded H.testis tissue reacted with CBX8 Antibody (Center) (Cat#AP21434c).
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, E
Primary Accession Q9HC52
Reactivity Human, Rat
Host Rabbit
Clonality polyclonal
Isotype Rabbit Ig
Calculated MW 43396 Da
Additional info
Gene ID 57332
Other Names Chromobox protein homolog 8, Polycomb 3 homolog, Pc3, hPc3, Rectachrome 1, CBX8, PC3, RC1
Target/Specificity This CBX8 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 197-230 amino acids from the Central region of human CBX8.
Dilution WB~~1:8000
IHC-P~~1:100
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCBX8 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CBX8
Synonyms PC3, RC1
Function Component of a Polycomb group (PcG) multiprotein PRC1- like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility.
Cellular Location Nucleus.
Research Areas

BACKGROUND

Component of a Polycomb group (PcG) multiprotein PRC1- like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility.

REFERENCES

Bardos J.I.,et al.J. Biol. Chem. 275:28785-28792(2000).
Michael M.Z.,et al.Submitted (DEC-2002) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Garcia-Cuellar M.P.,et al.Oncogene 20:411-419(2001).
Levine S.S.,et al.Mol. Cell. Biol. 22:6070-6078(2002).

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