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SPHK2 Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - SPHK2 Antibody (N-term) AP21357a
    Anti-SPHK2 Antibody (N-term)at 1:1000 dilution + human pancreas lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 69 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - SPHK2 Antibody (N-term) AP21357a
    Anti-SPHK2 Antibody (N-term)at 1:2000 dilution + human liver lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 69 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, E
Primary Accession Q9NRA0
Reactivity Human
Host Rabbit
Clonality polyclonal
Isotype Rabbit Ig
Calculated MW 69217 Da
Additional info
Gene ID 56848
Other Names Sphingosine kinase 2, SK 2, SPK 2, SPHK2
Target/Specificity This SPHK2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 52-84 amino acids from the N-terminal region of human SPHK2.
Dilution WB~~1:2000
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSPHK2 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SPHK2
Function Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions. Also acts on D-erythro- dihydrosphingosine, D-erythro-sphingosine and L-threo- dihydrosphingosine. Binds phosphoinositides.
Cellular Location Isoform 1: Cytoplasm. Membrane.
Research Areas

BACKGROUND

Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions. Also acts on D-erythro- dihydrosphingosine, D-erythro-sphingosine and L-threo- dihydrosphingosine. Binds phosphoinositides.

REFERENCES

Liu H.,et al.J. Biol. Chem. 275:19513-19520(2000).
Alemany R.,et al.Submitted (NOV-2006) to the EMBL/GenBank/DDBJ databases.
Wiemann S.,et al.Genome Res. 11:422-435(2001).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Grimwood J.,et al.Nature 428:529-535(2004).

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