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NRXN1 Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 14 - NRXN1 Antibody (N-term) AP21335a
    AP21335a staining NRXN1 in human brain sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
  • 1 - NRXN1 Antibody (N-term) AP21335a
    All lanes : Anti-NRXN1 Antibody (N-term) at 1:2000 dilution Lane 1: SH-SY5Y whole cell lysates Lane 2: human cerebellum lysates Lane 3: mouse brain lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 47 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IHC-P, WB, E
Primary Accession P58400
Reactivity Human, Mouse
Host Rabbit
Clonality polyclonal
Isotype Rabbit Ig
Calculated MW 46861 Da
Additional info
Gene ID 9378
Other Names Neurexin-1-beta, Neurexin I-beta, NRXN1
Target/Specificity This NRXN1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 77-110 amino acids from the N-terminal region of human NRXN1.
Dilution IHC-P~~1:25
WB~~1:2000
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsNRXN1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NRXN1
Function Neuronal cell surface protein that may be involved in cell recognition and cell adhesion by forming intracellular junctions through binding to neuroligins. May play a role in formation or maintenance of synaptic junctions. May mediate intracellular signaling. May play a role in angiogenesis (By similarity).
Cellular Location Cell membrane; Single-pass type I membrane protein. Cell junction, synapse. Note=Localized on the pre- synaptic membrane.
Research Areas

BACKGROUND

Neuronal cell surface protein that may be involved in cell recognition and cell adhesion by forming intracellular junctions through binding to neuroligins. May play a role in formation or maintenance of synaptic junctions. May mediate intracellular signaling. May play a role in angiogenesis (By similarity).

REFERENCES

Kleiderlein J.J.,et al.Hum. Genet. 103:666-673(1998).
Hillier L.W.,et al.Nature 434:724-731(2005).
Chen X.,et al.Nat. Struct. Mol. Biol. 15:50-56(2008).

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