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>   首页   >   产品   >   一抗   >   发育生物学   >   (Mouse) Pou5f1 Antibody (Center)   

(Mouse) Pou5f1 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - (Mouse) Pou5f1 Antibody (Center) AP21283c
    All lanes : Anti-Pou5f1 Antibody (Center) at 1:4000 dilution Lane 1: F9 whole cell lysates Lane 2: NCCIT whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 38 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, E
Primary Accession P20263
Reactivity Human, Mouse
Host Rabbit
Clonality polyclonal
Isotype Rabbit Ig
Calculated MW 38216 Da
Additional info
Gene ID 18999
Other Names POU domain, class 5, transcription factor 1, NF-A3, Octamer-binding protein 3, Oct-3, Octamer-binding protein 4, Oct-4, Octamer-binding transcription factor 3, OTF-3, Pou5f1, Oct-3, Oct-4, Otf-3, Otf3
Target/Specificity This Mouse Pou5f1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 214-247 amino acids from the Central region of Mouse Pou5f1.
Dilution WB~~1:4000
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions(Mouse) Pou5f1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name Pou5f1
Synonyms Oct-3, Oct-4, Otf-3, Otf3
Function Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency.
Cellular Location Cytoplasm. Nucleus {ECO:0000255|PROSITE-ProRule:PRU00108, ECO:0000255|PROSITE- ProRule:PRU00530, ECO:0000269|PubMed:17496161, ECO:0000269|PubMed:17525163}. Note=Expressed in a diffuse and slightly punctuate pattern
Tissue Location Expressed the totipotent and pluripotent stem cells of the pregastrulation embryo. Also expressed in primordial germ cells and in the female germ line. Absent from adult tissues
Research Areas

BACKGROUND

Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency.

REFERENCES

Rosner M.H.,et al.Nature 345:686-692(1990).
Schoeler H.R.,et al.Nature 344:435-439(1990).
Okamoto K.,et al.Cell 60:461-472(1990).
Okazawa H.,et al.EMBO J. 10:2997-3005(1991).
Tsuruzoe S.,et al.Biochem. Biophys. Res. Commun. 351:920-926(2006).

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