IL24 Antibody

Purified Rabbit Polyclonal Antibody (Pab)

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AP21241a staining IL24 in human melanoma sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
Overlay histogram showing U-2 OS cells stained with AP21241a (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP21241a, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit lgG (H+L) (1583138) at 1/400 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
All lanes : Anti-IL24 Antibody at 1:2000 dilution Lane 1: Hela whole cell lysates Lane 2: Jurkat whole cell lysates Lane 3: K562 whole cell lysates Lane 4: A431 whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 24 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

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Product info
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	IHC-P, WB, FC, E
Primary Accession	Q13007
Reactivity	Human
Host	Rabbit
Clonality	polyclonal
Isotype	Rabbit Ig
Calculated MW	23825 Da

Additional info
Gene ID	11009
Other Names	Interleukin-24, IL-24, Melanoma differentiation-associated gene 7 protein, MDA-7, Suppression of tumorigenicity 16 protein, IL24, MDA7, ST16
Target/Specificity	This IL24 antibody is generated from a rabbit immunized with a recombinant protein.
Dilution	IHC-P~~1:25 FC~~1:25 WB~~1:2000
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	IL24 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	IL24
Synonyms	MDA7, ST16
Function	Has antiproliferative properties on melanoma cells and may contribute to terminal cell differentiation.
Cellular Location	Secreted.
Tissue Location	Up-regulated in melanoma cells induced to terminally differentiate

Research Areas

BACKGROUND

Has antiproliferative properties on melanoma cells and may contribute to terminal cell differentiation.

REFERENCES

Jiang H.,et al.Oncogene 11:2477-2486(1995).
Peat J.,et al.Submitted (JUN-2000) to the EMBL/GenBank/DDBJ databases.
Huang E.Y.,et al.Oncogene 20:7051-7063(2001).
Allen M.,et al.J. Invest. Dermatol. 123:583-588(2004).
Allen M.,et al.Int. J. Immunogenet. 32:375-378(2005).

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Cat# AP21241a

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IL24 Antibody

Purified Rabbit Polyclonal Antibody (Pab)

BACKGROUND

REFERENCES

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