|Application ||WB, E|
|Other Accession||P60605, Q17QG5, NP_003334|
|Other Names||Ubiquitin-conjugating enzyme E2 G2, Ubiquitin carrier protein G2, Ubiquitin-protein ligase G2, UBE2G2|
|Target/Specificity||This UBE2G2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 17-47 amino acids from the N-terminal region of human UBE2G2.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||UBE2G2 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys- 48'-linked polyubiquitination. Involved in endoplasmic reticulum- associated degradation (ERAD).|
Provided below are standard protocols that you may find useful for product applications.
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBE2G2 is a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein shares 100% sequence identity with the mouse counterpart. This gene is ubiquitously expressed, with high expression seen in adult muscle.
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Rose, S.A., et al., Cytogenet. Cell Genet. 83 (1-2), 98-99 (1998).