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ATP2B3 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 3 - ATP2B3 Antibody (C-term) AP21234b
    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Hela (Human Cervical epithelial adenocarcinoma cell line) cells labeling ATP2B3 with AP21234b at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-rabbit IgG (NK179883) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing membrane staining on Hela cell line.The nuclear counter stain is DAPI (blue).
  • 1 - ATP2B3 Antibody (C-term) AP21234b
    Anti-ATP2B3 Antibody (C-term)at 1:2000 dilution + U-87 MG whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 134 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IF, WB, E
Primary Accession Q16720
Reactivity Human
Host Rabbit
Clonality polyclonal
Isotype Rabbit Ig
Calculated MW 134197 Da
Additional info
Gene ID 492
Target/Specificity This ATP2B3 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 1161-1194 amino acids from the C-terminal region of human ATP2B3.
Dilution IF~~1:25
WB~~1:2000
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsATP2B3 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ATP2B3
Function This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium out of the cell.
Cellular Location Cell membrane; Multi-pass membrane protein.
Tissue Location Highly expressed in the cerebellum, particulary in the presynaptic terminals of parallel fibers- Purkinje neurons. Isoform XE and isoform XB are the most abundant isoforms and are detected at low levels in brain and fetal skeletal muscle. The other isoforms are only found at lower levels and not in fetal tissues.
Research Areas
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