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(Mouse) Nr4a2 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 14 - (Mouse) Nr4a2 Antibody (Center) AP21233c
    AP21233c staining (Mouse) Nr4a2 in mouse spleen sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
  • 14 - (Mouse) Nr4a2 Antibody (Center) AP21233c
    AP21233c staining (Mouse) Nr4a2 in mouse brain sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
  • 1 - (Mouse) Nr4a2 Antibody (Center) AP21233c
    All lanes : Anti-Nr4a2 Antibody (Center) at 1:2000 dilution Lane 1: mouse brain lysates Lane 2: rat brain lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 67 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - (Mouse) Nr4a2 Antibody (Center) AP21233c
    Anti-Nr4a2 Antibody (Center)at 1:2000 dilution + mouse pancreas lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 67 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IHC-P, WB, E
Primary Accession Q06219
Reactivity Mouse, Rat
Host Rabbit
Clonality polyclonal
Isotype Rabbit Ig
Calculated MW 66593 Da
Additional info
Gene ID 18227
Target/Specificity This mouse Nr4a2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 164-197 amino acids from the Central region of mouse Nr4a2.
Dilution IHC-P~~1:25
WB~~1:2000
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions(Mouse) Nr4a2 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name Nr4a2
Synonyms Nurr1
Function Transcriptional regulator which is important for the differentiation and maintenance of meso-diencephalic dopaminergic (mdDA) neurons during development. It is crucial for expression of a set of genes such as SLC6A3, SLC18A2, TH and DRD2 which are essential for development of mdDA neurons.
Cellular Location Cytoplasm. Nucleus. Note=Mostly nuclear; oxidative stress promotes cytoplasmic localization.
Tissue Location Brain.
Research Areas
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