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STAT5A Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 14 - STAT5A Antibody (C-term) AP21232b
    AP21232b staining STAT5A in Human thymus tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
  • 1 - STAT5A Antibody (C-term) AP21232b
    All lanes : Anti-STAT5A Antibody (C-term) at 1:2000 dilution Lane 1: K562 whole cell lysates Lane 2: L929 whole cell lysates Lane 3: PC-12 whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 91 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - STAT5A Antibody (C-term) AP21232b
    All lanes : Anti-STAT5A Antibody (C-term) at 1:2000 dilution Lane 1: K562 whole cell lysates Lane 2: L929 whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 91 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, E
Primary Accession P42229
Reactivity Human, Mouse
Host Rabbit
Clonality polyclonal
Isotype Rabbit Ig
Calculated MW 90647 Da
Additional info
Gene ID 6776
Target/Specificity This STAT5A antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 763-797 amino acids from the C-terminal region of human STAT5A.
Dilution IHC-P~~1:25
WB~~1:2000
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSTAT5A Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name STAT5A
Synonyms STAT5
Function Carries out a dual function: signal transduction and activation of transcription. Mediates cellular responses to the cytokine KITLG/SCF and other growth factors. Mediates cellular responses to ERBB4. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Binds to the GAS element and activates PRL-induced transcription. Regulates the expression of milk proteins during lactation.
Cellular Location Cytoplasm. Nucleus. Note=Translocated into the nucleus in response to phosphorylation
Research Areas
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