CHST1 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

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AP21231b staining CHST1 in Human skeletal muscle tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
All lanes : Anti-CHST1 Antibody (C-term) at 1:2000 dilution Lane 1: human brain lysates Lane 2: Hela whole cell lysates Lane 3: Jurkat whole cell lysates Lane 4: mouse brain lysates Lane 5: rat brain lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 47 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

Product info
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P, E
Primary Accession	O43916
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	polyclonal
Isotype	Rabbit Ig
Calculated MW	46715 Da

Additional info
Gene ID	8534
Target/Specificity	This CHST1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 392-426 amino acids from the C-terminal region of human CHST1.
Dilution	IHC-P~~1:25 WB~~1:2000
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	CHST1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	CHST1
Function	Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of galactose (Gal) residues of keratan. Has a preference for sulfating keratan sulfate, but it also transfers sulfate to the unsulfated polymer. The sulfotransferase activity on sialyl LacNAc structures is much higher than the corresponding desialylated substrate, and only internal Gal residues are sulfated. May function in the sulfation of sialyl N- acetyllactosamine oligosaccharide chains attached to glycoproteins. Participates in biosynthesis of selectin ligands. Selectin ligands are present in high endothelial cells (HEVs) and play a central role in lymphocyte homing at sites of inflammation.
Cellular Location	Golgi apparatus membrane; Single-pass type II membrane protein
Tissue Location	Widely expressed at low level. Expressed in brain and skeletal muscle. Expressed by high endothelial cells (HEVs) and leukocytes.

Research Areas

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Cat# AP21231b

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