注册 | 登录
所有产品
  • 所有产品
  • 一抗
  • 裂解液
>   首页   >   产品   >   一抗   >   其他   >   Mouse Rad9a Antibody (N-term)   

Mouse Rad9a Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - Mouse Rad9a Antibody (N-term) AP21218a
    Anti-Rad9a Antibody (N-term) at 1:2000 dilution + M. brain tissue lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 42 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - Mouse Rad9a Antibody (N-term) AP21218a
    Anti-Rad9a Antibody (N-term) at 1:2000 dilution + M. brain tissue lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 42 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 产品详情
  • 实验流程
  • 背景知识
Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, E
Primary Accession Q9Z0F6
Reactivity Mouse
Host Rabbit
Clonality polyclonal
Isotype Rabbit Ig
Calculated MW 42059 Da
Additional info
Gene ID 19367
Other Names Cell cycle checkpoint control protein RAD9A, mRAD9, DNA repair exonuclease rad9 homolog A, Rad9-like protein, Rad9a, Rad9
Target/Specificity This mouse Rad9a antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 29-62 amino acids from the N-terminal region of mouse Rad9a.
Dilution WB~~1:2000
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMouse Rad9a Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name Rad9a
Synonyms Rad9
Function Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates. The 9-1-1 complex is necessary for the recruitment of RHNO1 to sites of double-stranded breaks (DSB) occurring during the S phase. RAD9A possesses 3'->5' double stranded DNA exonuclease activity (By similarity).
Cellular Location Nucleus.
Tissue Location Expressed in heart, brain, spleen, lung, liver, skeletal muscle, kidney and testis
Research Areas

BACKGROUND

Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates. The 9-1-1 complex is necessary for the recruitment of RHNO1 to sites of double-stranded breaks (DSB) occurring during the S phase. RAD9A possesses 3'->5' double stranded DNA exonuclease activity (By similarity).

REFERENCES

Hang H.,et al.J. Cell. Physiol. 177:241-247(1998).
Carninci P.,et al.Science 309:1559-1563(2005).
Park Y.-G.,et al.Submitted (JAN-2002) to the EMBL/GenBank/DDBJ databases.
Ishii H.,et al.Proc. Natl. Acad. Sci. U.S.A. 102:9655-9660(2005).
Sweet S.M.,et al.Mol. Cell. Proteomics 8:904-912(2009).

FeedBack

终于等到您。感谢您使用ABGENT(百奇生物)抗体产品。
点击下方“我要评价 ”按钮提交您的反馈信息,您的反馈和评价是我们最宝贵的财富之一,
我们将在1-3个工作日内处理您的反馈信息。

如有疑问,联系:0512-69552906 tech.abgent@wuxiapptec.com.


我要评价