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>   首页   >   产品   >   一抗   >   神经科学   >   AVPR2 Antibody (C-term)   

AVPR2 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 4 - AVPR2 Antibody (C-term) AP21193b
    Overlay histogram showing Jurkat cells stained with AP21193b (green line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody ( , 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit lgG (H+L) (1583138) at 1/400 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • 1 - AVPR2 Antibody (C-term) AP21193b
    All lanes : Anti-AVPR2 Antibody (C-term) at 1:2000 dilution Lane 1: Hela whole cell lysates Lane 2: Jurkat whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 40 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, FC, E
Primary Accession P30518
Reactivity Human
Host Rabbit
Clonality polyclonal
Isotype Rabbit Ig
Calculated MW 40279 Da
Additional info
Gene ID 554
Other Names Vasopressin V2 receptor, V2R, AVPR V2, Antidiuretic hormone receptor, Renal-type arginine vasopressin receptor, AVPR2, ADHR, DIR, DIR3, V2R
Target/Specificity This AVPR2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 343-377 amino acids from the C-terminal region of human AVPR2.
Dilution FC~~1:25
WB~~1:2000
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsAVPR2 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name AVPR2
Synonyms ADHR, DIR, DIR3, V2R
Function Receptor for arginine vasopressin. The activity of this receptor is mediated by G proteins which activate adenylate cyclase. Involved in renal water reabsorption.
Cellular Location Cell membrane; Multi-pass membrane protein.
Tissue Location Kidney.
Research Areas

BACKGROUND

Receptor for arginine vasopressin. The activity of this receptor is mediated by G proteins which activate adenylate cyclase. Involved in renal water reabsorption.

REFERENCES

Seibold A.,et al.Am. J. Hum. Genet. 51:1078-1083(1992).
Birnbaumer M.,et al.Nature 357:333-335(1992).
Wildin R.S.,et al.Am. J. Hum. Genet. 55:266-277(1994).
Fay M.J.,et al.Peptides 17:477-481(1996).
North W.G.,et al.Cancer Res. 58:1866-1871(1998).

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