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>   首页   >   产品   >   一抗   >   癌症   >   FGF9 Antibody   

FGF9 Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 4 - FGF9 Antibody AP21143a
    Overlay histogram showing MCF-7 cells stained with AP21143a (green line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody ( , 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit lgG (H+L) (1583138) at 1/400 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • 2 - FGF9 Antibody AP21143a
    AP21143a staining FGF9 in Human liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
  • 1 - FGF9 Antibody AP21143a
    All lanes : Anti-FGF9 Antibody at 1:4000 dilution Lane 1: human brain lysates Lane 2: human kidney lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 23 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - FGF9 Antibody AP21143a
    All lanes : Anti-FGF9 Antibody at 1:4000 dilution Lane 1: SW480 whole cell lysates Lane 2: mouse kidney lysates Lane 3: human brain lysates Lane 4: human kidney lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 23 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC, FC, E
Primary Accession P31371
Reactivity Human, Mouse
Host Rabbit
Clonality polyclonal
Isotype Rabbit Ig
Calculated MW 23441 Da
Additional info
Gene ID 2254
Other Names Fibroblast growth factor 9, FGF-9, Glia-activating factor, GAF, Heparin-binding growth factor 9, HBGF-9, FGF9
Target/Specificity This FGF9 antibody is generated from a rabbit immunized with a recombinant protein of human FGF9.
Dilution FC~~1:25
IHC~~1:25
WB~~1:4000
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsFGF9 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name FGF9
Function Plays an important role in the regulation of embryonic development, cell proliferation, cell differentiation and cell migration. May have a role in glial cell growth and differentiation during development, gliosis during repair and regeneration of brain tissue after damage, differentiation and survival of neuronal cells, and growth stimulation of glial tumors.
Cellular Location Secreted.
Tissue Location Glial cells.
Research Areas

BACKGROUND

Plays an important role in the regulation of embryonic development, cell proliferation, cell differentiation and cell migration. May have a role in glial cell growth and differentiation during development, gliosis during repair and regeneration of brain tissue after damage, differentiation and survival of neuronal cells, and growth stimulation of glial tumors.

REFERENCES

Miyamoto M.,et al.Mol. Cell. Biol. 13:4251-4259(1993).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Dunham A.,et al.Nature 428:522-528(2004).
Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
Naruo K.,et al.J. Biol. Chem. 268:2857-2864(1993).

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