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>   首页   >   产品   >   一抗   >   精选抗体   >   G蛋白偶联受体抗体(GPCR)   >   ADORA2A Antibody (C-term)   

ADORA2A Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 4 - ADORA2A Antibody (C-term) AP21140a
    Overlay histogram showing SH-SY5Y cells stained with AP21140a (green line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP21140a, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit lgG (H+L) (1583138) at 1/400 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • 14 - ADORA2A Antibody (C-term) AP21140a
    AP21140a staining ADORA2A in Human heart tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
  • 1 - ADORA2A Antibody (C-term) AP21140a
    Western blot analysis of lysate from SH-SY5Y cell line, using ADORA2A Antibody (C-term)(Cat. #AP21140a). AP21140a was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, FC, E
Primary Accession P29274
Reactivity Human
Host Rabbit
Clonality polyclonal
Isotype Rabbit Ig
Calculated MW 44707 Da
Additional info
Gene ID 135
Other Names Adenosine receptor A2a, ADORA2A, ADORA2
Target/Specificity This ADORA2A antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 347-381 amino acids from the C-terminal region of human ADORA2A.
Dilution FC~~1:25
IHC-P~~1:25
WB~~1:1000
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsADORA2A Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ADORA2A
Synonyms ADORA2
Function Receptor for adenosine. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase.
Cellular Location Cell membrane; Multi-pass membrane protein.
Research Areas

BACKGROUND

Receptor for adenosine. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase.

REFERENCES

Tiffany H.L.,et al.Submitted (JUL-1992) to the EMBL/GenBank/DDBJ databases.
Salvatore C.A.,et al.Submitted (SEP-1992) to the EMBL/GenBank/DDBJ databases.
Furlong T.J.,et al.Brain Res. Mol. Brain Res. 15:62-66(1992).
Le F.,et al.Biochem. Biophys. Res. Commun. 223:461-467(1996).
Puhl H.L. III,et al.Submitted (JUL-2002) to the EMBL/GenBank/DDBJ databases.

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