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>   首页   >   产品   >   一抗   >   干细胞   >   (Mouse) Scx Antibody (N-term)   

(Mouse) Scx Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 14 - (Mouse) Scx Antibody (N-term) AP21125a
    AP21125a staining Scx in Mouse lung tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
  • 1 - (Mouse) Scx Antibody (N-term) AP21125a
    Western blot analysis of lysate from mouse lung tissue lysate, using Scx Antibody (N-term)(Cat. #AP21125a). AP21125a was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, E
Primary Accession Q64124
Reactivity Mouse
Host Rabbit
Clonality polyclonal
Isotype Rabbit Ig
Calculated MW 22239 Da
Additional info
Gene ID 20289
Other Names Basic helix-loop-helix transcription factor scleraxis, Scx
Target/Specificity This mouse Scx antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 69-102 amino acids from the N-terminal region of mouse Scx.
Dilution IHC-P~~1:25
WB~~1:1000
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions(Mouse) Scx Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name Scx
Function Plays an early essential role in mesoderm formation, as well as a later role in formation of somite-derived chondrogenic lineages.
Cellular Location Nucleus {ECO:0000255|PROSITE- ProRule:PRU00981}
Tissue Location Expressed in mesenchymal precursors of cartilage and in connective tissue. Highly expressed in tendons in the limb, tongue and diaphragm and in cartilage of the bronchi
Research Areas

BACKGROUND

Plays an early essential role in mesoderm formation, as well as a later role in formation of somite-derived chondrogenic lineages.

REFERENCES

Cserjesi P.,et al.Development 121:1099-1110(1995).
Brown D.,et al.Development 126:4317-4329(1999).

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