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UBE4B Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - UBE4B Antibody (N-term) AP2111a
    All lanes : Anti-UBE4B Antibody (N-term) at 1:1000 dilution Lane 1: K562 whole cell lysate Lane 2: Raji whole cell lysate Lane 3: Hela whole cell lysate Lane 4: NIH/3T3 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size :146kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - UBE4B Antibody (N-term) AP2111a
    The anti-UBE4B Pab (Cat. #AP2111a) is used in Western blot to detect UBE4B in mouse kidney tissue lysate.
  • 14 - UBE4B Antibody (N-term) AP2111a
    Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, E
Primary Accession O95155
Other Accession Q9ES00
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Additional info
Gene ID 10277
Other Names Ubiquitin conjugation factor E4 B, 632-, UBE4B (HGNC:12500)
Target/Specificity This UBE4B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 112-142 amino acids from the N-terminal region of human UBE4B.
Dilution WB~~1:1000
IHC-P~~1:50~100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsUBE4B Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name UBE4B (HGNC:12500)
Function Ubiquitin-protein ligase that probably functions as an E3 ligase in conjunction with specific E1 and E2 ligases. May also function as an E4 ligase mediating the assembly of polyubiquitin chains on substrates ubiquitinated by another E3 ubiquitin ligase.
Cellular Location Cytoplasm {ECO:0000250|UniProtKB:Q9ES00}. Nucleus {ECO:0000250|UniProtKB:Q9ES00}
Tissue Location Highest expression in ovary, testis, heart and skeletal muscle. Expression is low in colon, thymus and peripheral blood leukocytes. Almost undetectable in lung and spleen
Research Areas

BACKGROUND

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBE4B represents an additional conjugation factor, E4, which is involved in multiubiquitin chain assembly. UBE4B also the strongest candidate among the neuroblastoma tumor suppressors.

REFERENCES

Ishikawa, K., et al., DNA Res. 5(3):169-176 (1998).
Mahoney, J.A., et al., Biochem. J. 361 (Pt 3), 587-595 (2002).

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