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>   首页   >   产品   >   一抗   >   精选抗体   >   (Mouse) Suz12 Antibody (Center)   

(Mouse) Suz12 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - (Mouse) Suz12 Antibody (Center) AP21111a
    All lanes : Anti-Suz12 Antibody (Center) at 1:1000 dilution Lane 1: F9 whole cell lysates Lane 2: Hela whole cell lysates Lane 3: MCF-7 whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 83 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - (Mouse) Suz12 Antibody (Center) AP21111a
    Western blot analysis of lysate from mouse F9 cell line, using Suz12 Antibody (Center)(Cat. #AP21111a). AP21111a was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, E
Primary Accession Q80U70
Reactivity Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 83026 Da
Additional info
Gene ID 52615
Other Names Polycomb protein Suz12, Suppressor of zeste 12 protein homolog, Suz12, D11Ertd530e, Kiaa0160
Target/Specificity This Mouse Suz12 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 496-530 amino acids from the Central region of Mouse Suz12.
Dilution WB~~1:1000
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions(Mouse) Suz12 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name Suz12
Synonyms D11Ertd530e, Kiaa0160
Function Polycomb group (PcG) protein. Component of the PRC2/EED- EZH2 complex, which methylates 'Lys-9' (H3K9me) and 'Lys-27' (H3K27me) of histone H3, leading to transcriptional repression of the affected target gene. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems (By similarity). Genes repressed by the PRC2/EED-EZH2 complex include HOXA7, HOXB6 and HOXC8.
Cellular Location Nucleus. Chromosome. Note=Localizes to the inactive X chromosome in trophoblast stem cells
Research Areas

BACKGROUND

Polycomb group (PcG) protein. Component of the PRC2/EED- EZH2 complex, which methylates 'Lys-9' (H3K9me) and 'Lys-27' (H3K27me) of histone H3, leading to transcriptional repression of the affected target gene. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems (By similarity). Genes repressed by the PRC2/EED-EZH2 complex include HOXA7, HOXB6 and HOXC8.

REFERENCES

Okazaki N.,et al.DNA Res. 10:35-48(2003).
Church D.M.,et al.PLoS Biol. 7:E1000112-E1000112(2009).
Pasini D.,et al.EMBO J. 23:4061-4071(2004).
Umlauf D.,et al.Nat. Genet. 36:1296-1300(2004).
Kuzmichev A.,et al.Proc. Natl. Acad. Sci. U.S.A. 102:1859-1864(2005).

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