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>   首页   >   产品   >   一抗   >   癌症   >   (Mouse) Eras Antibody (N-term)   

(Mouse) Eras Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 4 - (Mouse) Eras Antibody (N-term) AP21087a
    Overlay histogram showing F9 cells stained with AP21087a (red line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP21087a, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit lgG (H+L) (1583138) at 1/400 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • 1 - (Mouse) Eras Antibody (N-term) AP21087a
    Western blot analysis of lysate from mouse F9 cell line, using Eras Antibody (N-term)(Cat. #AP21087a). AP21087a was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, FC, E
Primary Accession Q7TN89
Reactivity Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 24321 Da
Additional info
Gene ID 353283
Other Names GTPase ERas, E-Ras, Embryonic stem cell-expressed Ras, Eras
Target/Specificity This Mouse Eras antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 19-52 amino acids from the N-terminal region of mouse Eras.
Dilution FC~~1:25
WB~~1:1000
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions(Mouse) Eras Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name Eras
Function Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. Plays an important role in the tumor-like growth properties of embryonic stem cells.
Cellular Location Cell membrane; Lipid-anchor; Cytoplasmic side
Tissue Location Expressed in several undifferentiated mouse embryonic stem cell lines.
Research Areas

BACKGROUND

Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. Plays an important role in the tumor-like growth properties of embryonic stem cells.

REFERENCES

Takahashi K.,et al.Nature 423:541-545(2003).
Carninci P.,et al.Science 309:1559-1563(2005).

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