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>   首页   >   产品   >   一抗   >   信号转导   >   CHMP4A Antibody (C-term)   

CHMP4A Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - CHMP4A Antibody (C-term) AP21067a
    Western blot analysis of lysates from human brain and human placenta tissue (from left to right), using CHMP4A Antibody (C-term)(Cat. #AP21067a). AP21067a was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, E
Primary Accession Q9BY43
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 25098 Da
Additional info
Gene ID 29082
Other Names Charged multivesicular body protein 4a, Chromatin-modifying protein 4a, CHMP4a, SNF7 homolog associated with Alix-2, SNF7-1, hSnf-1, Vacuolar protein sorting-associated protein 32-1, Vps32-1, hVps32-1, CHMP4A, C14orf123, SHAX2
Target/Specificity This CHMP4A antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 205-239 amino acids from the C-terminal region of human CHMP4A.
Dilution WB~~1:1000
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCHMP4A Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CHMP4A
Synonyms C14orf123, SHAX2
Function Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and the budding of enveloped viruses (HIV-1 and other lentiviruses). ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4. When overexpressed, membrane-assembled circular arrays of CHMP4A filaments can promote or stabilize negative curvature and outward budding. Via its interaction with PDCD6IP involved in HIV-1 p6- and p9-dependent virus release.
Cellular Location Cytoplasmic vesicle membrane. Late endosome membrane; Peripheral membrane protein Note=Membrane-associated. Localizes to large vesicle-like structures. Localizes to the midbody of dividing cells. Localized in two distinct rings on either side of the Fleming body
Tissue Location Widely expressed. Expressed at higher level in heart, kidney, liver and skeletal muscle. Also expressed in brain, placenta, lung and pancreas.
Research Areas

BACKGROUND

Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and the budding of enveloped viruses (HIV-1 and other lentiviruses). ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4. When overexpressed, membrane-assembled circular arrays of CHMP4A filaments can promote or stabilize negative curvature and outward budding. Via its interaction with PDCD6IP involved in HIV-1 p6- and p9-dependent virus release.

REFERENCES

Katoh K.,et al.J. Biol. Chem. 278:39104-39113(2003).
Peck J.W.,et al.Biochem. J. 377:693-700(2004).
Li Y.,et al.Submitted (DEC-1999) to the EMBL/GenBank/DDBJ databases.
Zhang Q.-H.,et al.Genome Res. 10:1546-1560(2000).
Li W.B.,et al.Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases.

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