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>   首页   >   产品   >   一抗   >   细胞生物学   >   SMURF1 Antibody (C-term)   

SMURF1 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - SMURF1 Antibody (C-term) AP2104B
    All lanes : Anti-SMURF1 Antibody (C-term) at 1:500 dilution Lane 1: MCF-7 whole cell lysate Lane 2: Hela whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 86 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 14 - SMURF1 Antibody (C-term) AP2104B
    Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, E
Primary Accession Q9HCE7
Other Accession A2A5Z6, Q9HAU4, Q9PUN2, Q9CUN6
Reactivity Human
Predicted Mouse, Xenopus
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Additional info
Gene ID 57154
Other Names E3 ubiquitin-protein ligase SMURF1, hSMURF1, 632-, SMAD ubiquitination regulatory factor 1, SMAD-specific E3 ubiquitin-protein ligase 1, SMURF1, KIAA1625
Target/Specificity This SMURF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 711-740 amino acids from the C-terminal region of human SMURF1.
Dilution WB~~1:500
IHC-P~~1:50~100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSMURF1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SMURF1
Synonyms KIAA1625
Function E3 ubiquitin-protein ligase that acts as a negative regulator of BMP signaling pathway. Mediates ubiquitination and degradation of SMAD1 and SMAD5, 2 receptor-regulated SMADs specific for the BMP pathway. Promotes ubiquitination and subsequent proteasomal degradation of TRAF family members and RHOA.
Cellular Location Cytoplasm. Cell membrane; Peripheral membrane protein; Cytoplasmic side
Research Areas
The interaction of mPar3 with the ubiquitin ligase Smurf2 is required for the establishment of neuronal polarity.
Author : Schwamborn JC, Khazaei MR, Püschel AW.
J Biol Chem. 2007 Nov 30;282(48):35259-68. Epub 2007 Sep 28.
17906294

BACKGROUND

SMURF1 is an E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. This protein interacts with receptor-regulated SMADs specific for the BMP pathway, SMAD1 and SMAD5, in order to trigger their ubiquitination and degradation and thereby their inactivation.

REFERENCES

Tajima, Y., et al., J. Biol. Chem. 278(12):10716-10721 (2003).
Suzuki, C., et al., J. Biol. Chem. 277(42):39919-39925 (2002).
Ebisawa, T., et al., J. Biol. Chem. 276(16):12477-12480 (2001).
Zhu, H., et al., Nature 400(6745):687-693 (1999).
Lambris, J., et al., J. Immunol. Methods 27(1):55-59 (1979).

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