- 文献引用 : 1
|Application ||IHC-P, WB, E|
|Other Accession||A2A5Z6, Q9HAU4, Q9PUN2, Q9CUN6|
|Other Names||E3 ubiquitin-protein ligase SMURF1, hSMURF1, 632-, SMAD ubiquitination regulatory factor 1, SMAD-specific E3 ubiquitin-protein ligase 1, SMURF1, KIAA1625|
|Target/Specificity||This SMURF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 711-740 amino acids from the C-terminal region of human SMURF1.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||SMURF1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||E3 ubiquitin-protein ligase that acts as a negative regulator of BMP signaling pathway. Mediates ubiquitination and degradation of SMAD1 and SMAD5, 2 receptor-regulated SMADs specific for the BMP pathway. Promotes ubiquitination and subsequent proteasomal degradation of TRAF family members and RHOA. Promotes ubiquitination and subsequent proteasomal degradation of MAVS (PubMed:23087404). Plays a role in dendrite formation by melanocytes (PubMed:23999003).|
|Cellular Location||Cytoplasm. Cell membrane; Peripheral membrane protein; Cytoplasmic side|
|Tissue Location||Expressed in melanocytes (PubMed:23999003).|
Author : Schwamborn JC, Khazaei MR, PÃ¼schel AW.
J Biol Chem. 2007 Nov 30;282(48):35259-68. Epub 2007 Sep 28.
Provided below are standard protocols that you may find useful for product applications.
SMURF1 is an E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. This protein interacts with receptor-regulated SMADs specific for the BMP pathway, SMAD1 and SMAD5, in order to trigger their ubiquitination and degradation and thereby their inactivation.
Tajima, Y., et al., J. Biol. Chem. 278(12):10716-10721 (2003).
Suzuki, C., et al., J. Biol. Chem. 277(42):39919-39925 (2002).
Ebisawa, T., et al., J. Biol. Chem. 276(16):12477-12480 (2001).
Zhu, H., et al., Nature 400(6745):687-693 (1999).
Lambris, J., et al., J. Immunol. Methods 27(1):55-59 (1979).