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>   首页   >   产品   >   一抗   >   发育生物学   >   (Mouse) Tdgf1 Antibody (N-term)   

(Mouse) Tdgf1 Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 4 - (Mouse) Tdgf1 Antibody (N-term) AP21040a
    Overlay histogram showing F9 cells stained with AP21040a (red line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP21040a, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit lgG (H+L) (1583138) at 1/400 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • 1 - (Mouse) Tdgf1 Antibody (N-term) AP21040a
    Western blot analysis of lysate from mouse F9 cell line, using (Mouse) Tdgf1 Antibody (N-term)(Cat. #AP21040a). AP21040a was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, FC, E
Primary Accession P51865
Reactivity Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 18754 Da
Additional info
Other Names Teratocarcinoma-derived growth factor, Cripto growth factor, Epidermal growth factor-like Cripto protein, Tdgf1, Cripto
Target/Specificity This Mouse Tdgf1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 37-70 amino acids from the N-terminal region of mouse Tdgf1.
Dilution FC~~1:25
WB~~1:1000
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions(Mouse) Tdgf1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name Tdgf1
Synonyms Cripto
Function Could play a role in the determination of the epiblastic cells that subsequently give rise to the mesoderm.
Cellular Location Cell membrane; Lipid-anchor, GPI-anchor
Tissue Location Expressed at low level in specific organs of the adult animal such as spleen, heart, lung and brain. During gastrulation, expressed in the forming mesoderm. In later stages of the developing heart, expression is restricted to the truncus arteriosus
Research Areas

BACKGROUND

Could play a role in the determination of the epiblastic cells that subsequently give rise to the mesoderm.

REFERENCES

Dono R.,et al.Development 118:1157-1168(1993).
Liguori G.,et al.Mamm. Genome 7:344-348(1996).
Minchiotti G.,et al.Mech. Dev. 90:133-142(2000).
Calvanese L.,et al.J. Med. Chem. 49:7054-7062(2006).

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