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>   首页   >   产品   >   一抗   >   其他   >   WDR82 Antibody (N-term)   

WDR82 Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 3 - WDR82 Antibody (N-term) AP20978b
    Fluorescent image of Hela cells stained with WDR82 Antibody (N-term)(Cat#AP20978b). AP20978b was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).
  • 1 - WDR82 Antibody (N-term) AP20978b
    Western blot analysis of lysates from NCCIT, SH-SY5Y cell line (from left to right), using WDR82 Antibody (N-term)(Cat. #AP20978b). AP20978b was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IF, E
Primary Accession Q6UXN9
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 35079 Da
Additional info
Gene ID 80335
Other Names WD repeat-containing protein 82, Protein TMEM113, Swd2, WDR82, TMEM113, WDR82A
Target/Specificity This WDR82 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 12~46 amino acids from the N-terminal region of human WDR82.
Dilution IF~~1:25
WB~~1:1000
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsWDR82 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name WDR82
Synonyms TMEM113, WDR82A
Function Regulatory component of the SET1 complex implicated in the tethering of this complex to transcriptional start sites of active genes. Facilitates histone H3 'Lys-4' methylation via recruitment of the SETD1A or SETD1B to the 'Ser-5' phosphorylated C-terminal domain (CTD) of RNA polymerase II large subunit (POLR2A). Component of PTW/PP1 phosphatase complex, which plays a role in the control of chromatin structure and cell cycle progression during the transition from mitosis into interphase.
Cellular Location Nucleus Note=Associates with chromatin
Research Areas

BACKGROUND

Regulatory component of the SET1 complex implicated in the tethering of this complex to transcriptional start sites of active genes. Facilitates histone H3 'Lys-4' methylation via recruitment of the SETD1A or SETD1B to the 'Ser-5' phosphorylated C-terminal domain (CTD) of RNA polymerase II large subunit (POLR2A). Component of PTW/PP1 phosphatase complex, which plays a role in the control of chromatin structure and cell cycle progression during the transition from mitosis into interphase.

REFERENCES

Clark H.F.,et al.Genome Res. 13:2265-2270(2003).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
Lee J.-H.,et al.J. Biol. Chem. 280:41725-41731(2005).
Higa L.A.,et al.Nat. Cell Biol. 8:1277-1283(2006).

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