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>   首页   >   产品   >   一抗   >   精选抗体   >   (RAT) Ccnd3 Antibody (N-term)   

(RAT) Ccnd3 Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - (RAT) Ccnd3 Antibody (N-term) AP20912b
    Western blot analysis of lysates from Hela cell line, mouse spleen, rat lung, rat stomach tissue (from left to right), using Ccnd3 Antibody (N-term)(Cat. #AP20912b). AP20912b was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.
  • 1 - (RAT) Ccnd3 Antibody (N-term) AP20912b
    Western blot analysis of lysate from rat spleen tissue, using Ccnd3 Antibody (N-term)(Cat. #AP20912b). AP20912b was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, E
Primary Accession P48961
Reactivity Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 32434 Da
Additional info
Gene ID 25193
Other Names G1/S-specific cyclin-D3, Ccnd3
Target/Specificity This rat Ccnd3 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 33-66 amino acids from the N-terminal region of rat Ccnd3.
Dilution WB~~1:500-1:1000
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions(RAT) Ccnd3 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name Ccnd3
Function Regulatory component of the cyclin D3-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D3/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex.
Cellular Location Nucleus. Cytoplasm. Membrane. Note=Cyclin D-CDK4 complexes accumulate at the nuclear membrane and are then translocated to the nucleus through interaction with KIP/CIP family members.
Research Areas

BACKGROUND

Regulatory component of the cyclin D3-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D3/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex.

REFERENCES

Hosokawa Y.,et al.Gene 147:249-252(1994).
Yang M.,et al.Gene 181:153-159(1996).

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