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>   首页   >   产品   >   一抗   >   心血管   >   EPHA4 Antibody (Center)   

EPHA4 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 14 - EPHA4 Antibody (Center) AP20902a
    Immunohistochemical analysis of paraffin-embedded H. testis section using EPHA4 Antibody (Center)(Cat#AP20902a). AP20902a was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
  • 1 - EPHA4 Antibody (Center) AP20902a
    Western blot analysis of lysates from Hela, MCF-7, SW480, mouse NIH/3T3 cell line, mouse brain tissue lysate (from left to right), using EPHA4 Antibody (Center)(Cat. #AP20902a). AP20902a was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, E
Primary Accession P54764
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 109860 Da
Additional info
Gene ID 2043
Other Names Ephrin type-A receptor 4, EPH-like kinase 8, EK8, hEK8, Tyrosine-protein kinase TYRO1, Tyrosine-protein kinase receptor SEK, EPHA4, HEK8, SEK, TYRO1
Target/Specificity This EPHA4 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 383-417 amino acids from the Central region of human EPHA4.
Dilution IHC-P~~1:25
WB~~1:1000
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsEPHA4 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name EPHA4
Synonyms HEK8, SEK, TYRO1
Function Receptor tyrosine kinase which binds membrane-bound ephrin family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Highly promiscuous, it has the unique property among Eph receptors to bind and to be physiologically activated by both GPI-anchored ephrin-A and transmembrane ephrin-B ligands including EFNA1 and EFNB3. Upon activation by ephrin ligands, modulates cell morphology and integrin-dependent cell adhesion through regulation of the Rac, Rap and Rho GTPases activity. Plays an important role in the development of the nervous system controlling different steps of axonal guidance including the establishment of the corticospinal projections. May also control the segregation of motor and sensory axons during neuromuscular circuit development. In addition to its role in axonal guidance plays a role in synaptic plasticity. Activated by EFNA1 phosphorylates CDK5 at 'Tyr-15' which in turn phosphorylates NGEF regulating RHOA and dendritic spine morphogenesis. In the nervous system, plays also a role in repair after injury preventing axonal regeneration and in angiogenesis playing a role in central nervous system vascular formation. Additionally, its promiscuity makes it available to participate in a variety of cell-cell signaling regulating for instance the development of the thymic epithelium.
Cellular Location Cell membrane; Single-pass type I membrane protein. Cell projection, axon. Cell projection, dendrite. Cell junction, synapse, postsynaptic cell membrane, postsynaptic density. Early endosome Note=Clustered upon activation and targeted to early endosome
Tissue Location Ubiquitous.
Research Areas

BACKGROUND

Receptor tyrosine kinase which binds membrane-bound ephrin family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Highly promiscuous, it has the unique property among Eph receptors to bind and to be physiologically activated by both GPI-anchored ephrin-A and transmembrane ephrin-B ligands including EFNA1 and EFNB3. Upon activation by ephrin ligands, modulates cell morphology and integrin-dependent cell adhesion through regulation of the Rac, Rap and Rho GTPases activity. Plays an important role in the development of the nervous system controlling different steps of axonal guidance including the establishment of the corticospinal projections. May also control the segregation of motor and sensory axons during neuromuscular circuit development. Beside its role in axonal guidance plays a role in synaptic plasticity. Activated by EFNA1 phosphorylates CDK5 at 'Tyr-15' which in turn phosphorylates NGEF regulating RHOA and dendritic spine morphogenesis. In the nervous system, plays also a role in repair after injury preventing axonal regeneration and in angiogenesis playing a role in central nervous system vascular formation. Additionally, its promiscuity makes it available to participate in a variety of cell-cell signaling regulating for instance the development of the thymic epithelium.

REFERENCES

Fox G.M.,et al.Oncogene 10:897-905(1995).
Richter M.,et al.J. Neurosci. 27:14205-14215(2007).
Fu W.Y.,et al.Nat. Neurosci. 10:67-76(2007).
Oppermann F.S.,et al.Mol. Cell. Proteomics 8:1751-1764(2009).
Qin H.,et al.J. Biol. Chem. 283:29473-29484(2008).

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