|Application ||WB, E|
|Calculated MW||87286 Da|
|Other Names||Transient receptor potential cation channel subfamily V member 6, TrpV6, CaT-like, CaT-L, Calcium transport protein 1, CaT1, Epithelial calcium channel 2, ECaC2, TRPV6, ECAC2|
|Target/Specificity||This TRPV6 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 352-385 amino acids from the Central region of human TRPV6.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||TRPV6 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Calcium selective cation channel that mediates Ca(2+) uptake in various tissues, including the intestine (PubMed:11097838, PubMed:11278579, PubMed:11248124 PubMed:15184369, PubMed:23612980, PubMed:29258289). Important for normal Ca(2+) ion homeostasis in the body, including bone and skin (By similarity). The channel is activated by low internal calcium level, probably including intracellular calcium store depletion, and the current exhibits an inward rectification (PubMed:15184369). Inactivation includes both a rapid Ca(2+)- dependent and a slower Ca(2+)-calmodulin-dependent mechanism; the latter may be regulated by phosphorylation. In vitro, is slowly inhibited by Mg(2+) in a voltage-independent manner. Heteromeric assembly with TRPV5 seems to modify channel properties. TRPV5- TRPV6 heteromultimeric concatemers exhibit voltage-dependent gating.|
|Cellular Location||Cell membrane; Multi-pass membrane protein|
|Tissue Location||Expressed at high levels in the gastrointestinal tract, including esophagus, stomach, duodenum, jejunum, ileum and colon, and in pancreas, placenta, prostate and salivary gland. Expressed at moderate levels in liver, kidney and testis. Expressed in trophoblasts of placenta villus trees (at protein level)(PubMed:23612980). Expressed in locally advanced prostate cancer, metastatic and androgen-insensitive prostatic lesions but not detected in healthy prostate tissue and benign prostatic hyperplasia.|
Provided below are standard protocols that you may find useful for product applications.
Calcium selective cation channel probably involved in Ca(2+) uptake in various tissues, including Ca(2+) reabsorption in intestine. The channel is activated by low internal calcium level, probably including intracellular calcium store depletion, and the current exhibits an inward rectification. Inactivation includes both, a rapid Ca(2+)-dependent and a slower Ca(2+)-calmodulin- dependent mechanism, the latter may be regulated by phosphorylation. In vitro, is slowly inhibited by Mg(2+) in a voltage-independent manner. Heteromeric assembly with TRPV5 seems to modify channel properties. TRPV5-TRPV6 heteromultimeric concatemers exhibit voltage-dependent gating (By similarity).
Peng J.-B.,et al.Biochem. Biophys. Res. Commun. 278:326-332(2000).
Wood R.J.,et al.BMC Physiol. 1:11-11(2001).
Peng J.-B.,et al.Genomics 76:99-109(2001).
Wissenbach U.,et al.J. Biol. Chem. 276:19461-19468(2001).
Peng J.-B.,et al.Submitted (MAR-2001) to the EMBL/GenBank/DDBJ databases.