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DFFB Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 14 - DFFB Antibody (Center) AP20612c
    Immunohistochemical analysis of paraffin-embedded M.kidney section using DFFB Antibody (Center)(Cat#AP20612c). AP20612c was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
  • 14 - DFFB Antibody (Center) AP20612c
    Immunohistochemical analysis of paraffin-embedded H.kidney section using DFFB Antibody (Center)(Cat#AP20612c). AP20612c was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
  • 4 - DFFB Antibody (Center) AP20612c
    Flow cytometric analysis of Hela cells using DFFB Antibody (Center)(green, Cat#AP20612c) compared to an isotype control of rabbit IgG(blue). AP20612c was diluted at 1:25 dilution. An Alexa Fluor® 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.
  • 1 - DFFB Antibody (Center) AP20612c
    Western blot analysis of lysate from A431 cell line, using DFFB Antibody (Center)(Cat. #AP20612c). AP20612c was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, FC, IHC-P, E
Primary Accession O76075
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 39110 Da
Additional info
Gene ID 1677
Other Names DNA fragmentation factor subunit beta, 3---, Caspase-activated deoxyribonuclease, CAD, Caspase-activated DNase, Caspase-activated nuclease, CPAN, DNA fragmentation factor 40 kDa subunit, DFF-40, DFFB, CAD, DFF2, DFF40
Target/Specificity This DFFB antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 203-238 amino acids from the Central region of human DFFB.
Dilution IHC-P~~1:25
FC~~1:25
WB~~1:1000
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsDFFB Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name DFFB
Synonyms CAD, DFF2, DFF40
Function Nuclease that induces DNA fragmentation and chromatin condensation during apoptosis. Degrades naked DNA and induces apoptotic morphology.
Cellular Location Cytoplasm. Nucleus.
Research Areas

BACKGROUND

Nuclease that induces DNA fragmentation and chromatin condensation during apoptosis. Degrades naked DNA and induces apoptotic morphology.

REFERENCES

Liu X.,et al.Proc. Natl. Acad. Sci. U.S.A. 95:8461-8466(1998).
Mukae N.,et al.Proc. Natl. Acad. Sci. U.S.A. 95:9123-9128(1998).
Halenbeck R.,et al.Curr. Biol. 8:537-540(1998).
Nakagawara A.,et al.Submitted (JUN-1999) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).

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