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CHML Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 4 - CHML Antibody (C-term) AP20592c
    Flow cytometric analysis of Hela cells using CHML Antibody (C-term)(green, Cat#AP20592c) compared to an isotype control of rabbit IgG(blue). AP20592c was diluted at 1:25 dilution. An Alexa Fluor® 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.
  • 14 - CHML Antibody (C-term) AP20592c
    Immunohistochemical analysis of paraffin-embedded H. stomach section using CHML Antibody (C-term)(Cat#AP20592c). AP20592c was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
  • 14 - CHML Antibody (C-term) AP20592c
    Immunohistochemical analysis of paraffin-embedded R. cerebellum section using CHML Antibody (C-term)(Cat#AP20592c). AP20592c was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
  • 14 - CHML Antibody (C-term) AP20592c
    Immunohistochemical analysis of paraffin-embedded M. cerebellum section using CHML Antibody (C-term)(Cat#AP20592c). AP20592c was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
  • 1 - CHML Antibody (C-term) AP20592c
    Western blot analysis of lysate from HT-1080 cell line, using CHML Antibody (C-term) (Cat. #AP20592c). AP20592c was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, FC, IHC-P, E
Primary Accession P26374
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 74071 Da
Additional info
Gene ID 1122
Other Names Rab proteins geranylgeranyltransferase component A 2, Choroideremia-like protein, Rab escort protein 2, REP-2, CHML, REP2
Target/Specificity This CHML antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 624-656 amino acids from the C-terminal region of human CHML.
Dilution FC~~1:25
IHC-P~~1:25
WB~~1:1000
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCHML Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CHML
Synonyms REP2
Function Substrate-binding subunit (component A) of the Rab geranylgeranyltransferase (GGTase) complex. Binds unprenylated Rab proteins and presents the substrate peptide to the catalytic component B. The component A is thought to be regenerated by transferring its prenylated Rab back to the donor membrane. Less effective than CHM in supporting prenylation of Rab3 family.
Cellular Location Cytoplasm, cytosol
Research Areas

BACKGROUND

Substrate-binding subunit (component A) of the Rab geranylgeranyltransferase (GGTase) complex. Binds unprenylated Rab proteins and presents the substrate peptide to the catalytic component B. The component A is thought to be regenerated by transferring its prenylated Rab back to the donor membrane. Less effective than CHM in supporting prenylation of Rab3 family.

REFERENCES

Cremers F.P.M.,et al.Hum. Mol. Genet. 1:71-75(1992).
Kasper G.,et al.Gene 295:27-32(2002).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Gregory S.G.,et al.Nature 441:315-321(2006).
Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.

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