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CYC1 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 3 - CYC1 Antibody (Center) AP20574c
    Fluorescent image of Hela cells stained with CYC1 Antibody (Center)(Cat#AP20574c). AP20574c was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).
  • 14 - CYC1 Antibody (Center) AP20574c
    Immunohistochemical analysis of paraffin-embedded H. stomach section using CYC1 Antibody (Center)(C-term)(Cat#AP20574c). AP20574c was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
  • 14 - CYC1 Antibody (Center) AP20574c
    Immunohistochemical analysis of paraffin-embedded M. stomach section using CYC1 Antibody (Center)(Cat#AP20574c). AP20574c was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
  • 14 - CYC1 Antibody (Center) AP20574c
    Immunohistochemical analysis of paraffin-embedded R. stomach section using CYC1 Antibody (Center)(Cat#AP20574c). AP20574c was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
  • 1 - CYC1 Antibody (Center) AP20574c
    Western blot analysis of lysates from A431, Hela, HepG2, MCF-7, U-251 MG cell lines and mouse brain tissue lysate (from left to right), using CYC1 Antibody (Center) (Cat. #AP20574c). AP20574c was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IF, IHC-P, E
Primary Accession P08574
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 35422 Da
Additional info
Gene ID 1537
Other Names Cytochrome c1, heme protein, mitochondrial, Complex III subunit 4, Complex III subunit IV, Cytochrome b-c1 complex subunit 4, Ubiquinol-cytochrome-c reductase complex cytochrome c1 subunit, Cytochrome c-1, CYC1
Target/Specificity This CYC1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 142-176 amino acids from the Central region of human CYC1.
Dilution IF~~1:25
IHC-P~~1:25
WB~~1:1000
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCYC1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CYC1
Function This is the heme-containing component of the cytochrome b-c1 complex, which accepts electrons from Rieske protein and transfers electrons to cytochrome c in the mitochondrial respiratory chain.
Cellular Location Mitochondrion inner membrane; Single-pass membrane protein; Intermembrane side
Research Areas

BACKGROUND

This is the heme-containing component of the cytochrome b-c1 complex, which accepts electrons from Rieske protein and transfers electrons to cytochrome c in the mitochondrial respiratory chain.

REFERENCES

Nishikimi M.,et al.Nucleic Acids Res. 16:3577-3577(1988).
Suzuki H.,et al.J. Biol. Chem. 264:1368-1374(1989).
Halleck A.,et al.Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
Kalnine N.,et al.Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases.
Nusbaum C.,et al.Nature 439:331-335(2006).

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