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HLA-B Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - HLA-B Antibody (N-term) AP20563a
    All lanes : Anti-HLA-B Antibody (N-term) at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: HL-60 whole cell lysate Lane 3: Jurkat whole cell lysate Lane 4: Ramos whole cell lysate Lane 5: human spleen lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 40 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - HLA-B Antibody (N-term) AP20563a
    All lanes : Anti-HLA-B Antibody (N-term) at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: HL-60 whole cell lysate Lane 3: A549 whole cell lysate Lane 4: Jurkat whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 40 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 14 - HLA-B Antibody (N-term) AP20563a
    AP20563a staining HLA-B in human tonsil tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, E
Primary Accession P30464
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Additional info
Other Names HLA class I histocompatibility antigen, B-15 alpha chain, MHC class I antigen B*15, HLA-B, HLAB
Target/Specificity This HLA-B antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 25-107 amino acids from the N-terminal region of human HLA-B.
Dilution WB~~1:1000
IHC-P~~1:25
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsHLA-B Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name HLA-B
Synonyms HLAB
Function Involved in the presentation of foreign antigens to the immune system.
Cellular Location Membrane; Single-pass type I membrane protein
Research Areas

BACKGROUND

Involved in the presentation of foreign antigens to the immune system.

REFERENCES

Little A.-M.,et al.Tissue Antigens 38:186-190(1991).
Hildebrand W.H.,et al.Tissue Antigens 43:209-218(1994).
Lin L.,et al.Tissue Antigens 47:265-274(1996).
Madrigal J.A.,et al.J. Immunol. 149:3411-3415(1992).
Watkins D.I.,et al.Nature 357:329-333(1992).

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