|Application ||WB, E|
|Calculated MW||29083 Da|
|Other Names||Zinc finger protein SNAI1, Protein snail homolog 1, Protein sna, SNAI1, SNAH|
|Target/Specificity||This SNAI1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 9-36 amino acids from the N-terminal region of human SNAI1.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||SNAI1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Involved in induction of the epithelial to mesenchymal transition (EMT), formation and maintenance of embryonic mesoderm, growth arrest, survival and cell migration. Binds to 3 E-boxes of the E-cadherin/CDH1 gene promoter and to the promoters of CLDN7 and KRT8 and, in association with histone demethylase KDM1A which it recruits to the promoters, causes a decrease in dimethylated H3K4 levels and represses transcription. During EMT, involved with LOXL2 in negatively regulating pericentromeric heterochromatin transcription (By similarity). SNAI1 recruits LOXL2 to pericentromeric regions to oxidize histone H3 and repress transcription which leads to release of heterochromatin component CBX5/HP1A, enabling chromatin reorganization and acquisition of mesenchymal traits (By similarity). Associates with EGR1 and SP1 to mediate tetradecanoyl phorbol acetate (TPA)-induced up- regulation of CDKN2B, possibly by binding to the CDKN2B promoter region 5'-TCACA-3. In addition, may also activate the CDKN2B promoter by itself.|
|Cellular Location||Nucleus. Cytoplasm. Note=Once phosphorylated (probably on Ser-107, Ser-111, Ser-115 and Ser-119) it is exported from the nucleus to the cytoplasm where subsequent phosphorylation of the destruction motif and ubiquitination involving BTRC occurs|
|Tissue Location||Expressed in a variety of tissues with the highest expression in kidney. Expressed in mesenchymal and epithelial cell lines.|
The Drosophila embryonic protein snail is a zinc finger transcriptional repressor which downregulates the expression of ectodermal genes within the mesoderm. The nuclear protein is structurally similar to the Drosophila snail protein, and is also thought to be critical for mesoderm formation in the developing embryo.
Strausberg, R.L., et al., Proc. Natl. Acad. Sci. U.S.A. 99(26):16899-16903 (2002).
Deloukas, P., et al., Nature 414(6866):865-871 (2001).
Batlle, E., et al., Nat. Cell Biol. 2(2):84-89 (2000).
Paznekas, W.A., et al., Genomics 62(1):42-49 (1999).
Twigg, S.R., et al., Hum. Genet. 105(4):320-326 (1999).