|Application ||WB, IHC-P, E|
|Other Names||Vesicle-associated membrane protein 8, VAMP-8, Endobrevin, EDB, VAMP8|
|Target/Specificity||This VAMP8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human VAMP8.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||VAMP8 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||SNAREs, Soluble N-ethylmaleimide-sensitive factor- attachment protein receptors, are essential proteins for fusion of cellular membranes. SNAREs localized on opposing membranes assemble to form a trans-SNARE complex, an extended, parallel four alpha-helical bundle that drives membrane fusion. VAMP8 is a SNARE involved in autophagy through the direct control of autophagosome membrane fusion with the lysososome membrane. Also required for dense-granule secretion in platelets. Plays also a role in regulated enzyme secretion in pancreatic acinar cells. Involved in the abscission of the midbody during cell division, which leads to completely separate daughter cells. Involved in the homotypic fusion of early and late endosomes.|
|Cellular Location||Lysosome membrane; Single-pass type IV membrane protein. Late endosome membrane; Single-pass type IV membrane protein Note=Perinuclear vesicular structures of the early and late endosomes, coated pits, and trans-Golgi. Sub-tight junctional domain in retinal pigment epithelium cells. Midbody region during cytokinesis. Lumenal oriented, apical membranes of nephric tubular cell. Cycles through the apical but not through the basolateral plasma membrane. Apical region of acinar cells; in zymogen granule membranes (By similarity).|
Involved in the targeting and/or fusion of transport vesicles to their target membrane. Involved for dense-granule secretion in platelets. Plays a role in regulated enzyme secretion in pancreatic acinar cells. Involved in the abscission of the midbody during cell division, which leads to completely separate daughter cells. Involved in the homotypic fusion of early and late endosomes (By similarity).
Wong S.H., et al. Mol. Biol. Cell 9:1549-1563(1998).
Kalnine N., et al. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
Ebert L., et al. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
Hillier L.W., et al. Nature 434:724-731(2005).
Polgar J., et al. Blood 100:1081-1083(2002).