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|Application ||WB, E|
|Calculated MW||34802 Da|
|Other Names||Zinc finger protein 42 homolog, Zfp-42, Reduced expression protein 1, REX-1, hREX-1, Zinc finger protein 754, ZFP42, REX1, ZNF754|
|Target/Specificity||This Rex1 (ZFP42) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human Rex1 (ZFP42).|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Rex1 (ZFP42) Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Involved in the reprogramming of X-chromosome inactivation during the acquisition of pluripotency. Required for efficient elongation of TSIX, a non-coding RNA antisense to XIST. Binds DXPas34 enhancer within the TSIX promoter. Involved in ES cell self-renewal (By similarity).|
|Tissue Location||Expressed in kidney, epidermal keratinocytes, prostate epithelial cells, bronchial and small airway lung epithelial cells (at protein level). Expressed in malignant kidney and several carcinoma cell lines (at protein level). Expressed in embryonic stem cells, kidney, epidermal keratinocytes, prostate epithelial cells, bronchial and small airway lung epithelial cells. Expressed in embryonal carcinomas, seminomas, malignant kidney and several carcinoma cell lines|
Author : Hewetson A, Chilton BS.
Mol Endocrinol. 2008 Apr;22(4):813-22. doi: 10.1210/me.2007-0432. Epub 2008 Jan 3.
Provided below are standard protocols that you may find useful for product applications.
Zinc finger proteins have regions (zinc finger domains) consisting of cysteines and histidines or cysteines alone which can form a tetrahedral complex around a Zinc ion. Zinc finger represent a class of DNA-binding proteins, act as transcriptional regulators of other genes. These multifunctional transcription factors exhibits control on a large number of cellular genes by binding to sites overlapping the transcription start site and plays an important role in development and differentiation. Hromas et al. in an effort to identify activators of the genetic cascade in hemopoietic differentiation probed a human myeloid cDNA library. ZNF42 may be a regulator of transcriptional events during hemopoietic development.
Hromas R, et al. J. Biol. Chem. 1991. 266: 14183-14187.
Morris J, et al. Blood 1995. 86: 3640-3647.