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BLMH Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - BLMH Antibody (Center) AP20513c
    All lanes : Anti-BLMH Antibody (Center) at 1:1000 dilution Lane 1: HL-60 whole cell lysate Lane 2: CCRF-CEM whole cell lysate Lane 3: SH-SY5Y whole cell lysate Lane 4: MOLT-4 whole cell lysate Lane 5: Neuro-2a whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 53 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - BLMH Antibody (Center) AP20513c
    All lanes : Anti-BLMH Antibody (Center) at 1:1000 dilution Lane 1: HL-60 whole cell lysate Lane 2: MOLT-4 whole cell lysate Lane 3: Neuro-2a whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 53 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 14 - BLMH Antibody (Center) AP20513c
    Immunohistochemical analysis of paraffin-embedded H. skin section using BLMH Antibody (Center)(Cat#AP20513C). AP20513C was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
  • 3 - BLMH Antibody (Center) AP20513c
    Fluorescent image of Hela cells stained with BLMH Antibody (Center)(Cat#AP20463C). AP20463C was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).
  • 1 - BLMH Antibody (Center) AP20513c
    BLMH Antibody (Center) (Cat. #AP20513c) western blot analysis in K562 cell line,mouse stomach and rat stomach tissue lysates (35ug/lane).This demonstrates the BLMH antibody detected the BLMH protein (arrow).
  • 1 - BLMH Antibody (Center) AP20513c
    BLMH Antibody (Center) (Cat. #AP20463c) western blot analysis in CEM,HL-60 cell line lysates (35ug/lane).This demonstrates the BLMH antibody detected the BLMH protein (arrow).
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IF, IHC-P, E
Primary Accession Q13867
Other Accession P70645, P13019, Q8R016
Reactivity Human, Mouse, Rat
Predicted Rabbit
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 52562 Da
Additional info
Gene ID 642
Other Names Bleomycin hydrolase, BH, BLM hydrolase, BMH, BLMH
Target/Specificity This BLMH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 212-242 amino acids from the Central region of human BLMH.
Dilution WB~~1:1000
IHC-P~~1:25
IF~~1:25
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsBLMH Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name BLMH
Function The normal physiological role of BLM hydrolase is unknown, but it catalyzes the inactivation of the antitumor drug BLM (a glycopeptide) by hydrolyzing the carboxamide bond of its B- aminoalaninamide moiety thus protecting normal and malignant cells from BLM toxicity.
Cellular Location Cytoplasm.
Research Areas

BACKGROUND

The normal physiological role of BLM hydrolase is unknown, but it catalyzes the inactivation of the antitumor drug BLM (a glycopeptide) by hydrolyzing the carboxamide bond of its B-aminoalaninamide moiety thus protecting normal and malignant cells from BLM toxicity (By similarity).

REFERENCES

Barrow I.K.-P., et al. Submitted (AUG-1998) to the EMBL/GenBank/DDBJ databases.
Ferrando A.A., et al. Cancer Res. 56:1746-1750(1996).
Broemme D., et al. Biochemistry 35:6706-6714(1996).
Kalnine N., et al. Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases.
Ota T., et al. Nat. Genet. 36:40-45(2004).

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