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PKP4 Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - PKP4 Antibody (C-term) AP20495b
    PKP4 Antibody (C-term) (Cat. #AP20495b) western blot analysis in 293,CEM,HL-60,K562 cell line and mouse kidney,lung tissue lysates (35ug/lane).This demonstrates the PKP4 antibody detected the PKP4 protein (arrow).
  • 14 - PKP4 Antibody (C-term) AP20495b
    Formalin-fixed and paraffin-embedded H.kidney tissue reacted with PKP4 Antibody (C-term) (Cat#AP20495b).
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, E
Primary Accession Q99569
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 131868 Da
Additional info
Gene ID 8502
Other Names Plakophilin-4, p0071, PKP4
Target/Specificity This PKP4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1040-1068 amino acids from the C-terminal region of human PKP4.
Dilution WB~~1:1000
IHC-P~~1:100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPKP4 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PKP4
Function Plays a role as a regulator of Rho activity during cytokinesis. May play a role in junctional plaques.
Cellular Location Cell junction, desmosome. Cytoplasm, cytoskeleton, spindle. Midbody. Cell membrane; Peripheral membrane protein. Note=Associated with the pericentrosomal region in interphase and with spindle poles during mitosis. In anaphase, during chromosome segregation, is recruited to the central microtubule bundle, focussed at the spindle midzone and ultimately localizes to the midbody at cytokinesis Constituent of the midbody cytoskeletal matrix. Colocalized with desmoplakin at desmosomal junctional plaques in cultured epithelial cells
Research Areas

BACKGROUND

Plays a role as a regulator of Rho activity during cytokinesis. May play a role in junctional plaques.

REFERENCES

Hatzfeld M., et al. J. Cell Sci. 109:2767-2778(1996).
Hillier L.W., et al. Nature 434:724-731(2005).
Salomon A.R., et al. Proc. Natl. Acad. Sci. U.S.A. 100:443-448(2003).
Zhang Y., et al. Mol. Cell. Proteomics 4:1240-1250(2005).
Tao W.A., et al. Nat. Methods 2:591-598(2005).

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