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ZRSR2 Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 14 - ZRSR2 Antibody (C-term) AP20436b
    Immunohistochemical analysis of paraffin-embedded H. prostate section using ZRSR2 Antibody (C-term)(Cat#AP20436b). AP20436b was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
  • 1 - ZRSR2 Antibody (C-term) AP20436b
    ZRSR2 Antibody (C-term) (Cat. #AP20436b) western blot analysis in 293,HepG2,HL-60,Jurkat,MCF-7,ZR-75-1 cell line lysates (35ug/lane).This demonstrates the ZRSR2 antibody detected the ZRSR2 protein (arrow).
  • 14 - ZRSR2 Antibody (C-term) AP20436b
    Formalin-fixed and paraffin-embedded H.tonsil tissue reacted with ZRSR2 Antibody (C-term) (Cat#AP20436b).
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, E
Primary Accession Q15696
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 58045 Da
Additional info
Gene ID 8233
Other Names U2 small nuclear ribonucleoprotein auxiliary factor 35 kDa subunit-related protein 2, CCCH type zinc finger, RNA-binding motif and serine/arginine rich protein 2, Renal carcinoma antigen NY-REN-20, U2(RNU2) small nuclear RNA auxiliary factor 1-like 2, U2AF35-related protein, URP, ZRSR2, U2AF1-RS2, U2AF1L2, U2AF1RS2, URP
Target/Specificity This ZRSR2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 453-482 amino acids from the C-terminal region of human ZRSR2.
Dilution IHC-P~~1:100
WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsZRSR2 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ZRSR2
Synonyms U2AF1-RS2, U2AF1L2, U2AF1RS2, URP
Function Pre-mRNA-binding protein required for splicing of both U2- and U12-type introns. Selectively interacts with the 3'-splice site of U2- and U12-type pre-mRNAs and promotes different steps in U2 and U12 intron splicing. Recruited to U12 pre-mRNAs in an ATP- dependent manner and is required for assembly of the prespliceosome, a precursor to other spliceosomal complexes. For U2-type introns, it is selectively and specifically required for the second step of splicing.
Cellular Location Nucleus.
Tissue Location Widely expressed.
Research Areas

BACKGROUND

Pre-mRNA-binding protein required for splicing of both U2-and U12-type introns. Selectively interacts with the 3'-splice site of U2-and U12-type pre-mRNAs and promotes different steps in U2 and U12 intron splicing. Recruited to U12 pre-mRNAs in an ATP-dependent manner and is required for assembly of the prespliceosome, a precursor to other spliceosomal complexes. For U2-type introns, it is selectively and specifically required for the second step of splicing.

REFERENCES

Kitagawa K., et al. Genomics 30:257-263(1995).
Ross M.T., et al. Nature 434:325-337(2005).
Tronchere H., et al. Nature 388:397-400(1997).
Scanlan M.J., et al. Int. J. Cancer 83:456-464(1999).
Will C.L., et al. RNA 10:929-941(2004).

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