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CCND3 Antibody (C-term S274)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - CCND3 Antibody (C-term S274) AP20418b
    CCND3 Antibody (C-term S274) (Cat. #AP20418b) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the CCND3 antibody detected the CCND3 protein (arrow).
  • 1 - CCND3 Antibody (C-term S274) AP20418b
    CCND3 Antibody (C-term S274) (Cat. #AP20418b) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the CCND3 antibody detected the CCND3 protein (arrow).
  • 14 - CCND3 Antibody (C-term S274) AP20418b
    Formalin-fixed and paraffin-embedded H.skin tissue reacted with CCND3 Antibody (C-term S274) (Cat#AP20418b).
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, E
Primary Accession P30281
Other Accession P30282, Q3MHH5
Reactivity Human, Mouse
Predicted Bovine
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 32520 Da
Additional info
Gene ID 896
Other Names G1/S-specific cyclin-D3, CCND3
Target/Specificity This CCND3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 252-281 amino acids from the C-terminal region of human CCND3.
Dilution WB~~1:1000
IHC-P~~1:100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCCND3 Antibody (C-term S274) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CCND3
Function Regulatory component of the cyclin D3-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D3/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex.
Cellular Location Nucleus. Cytoplasm. Membrane. Note=Cyclin D-CDK4 complexes accumulate at the nuclear membrane and are then translocated to the nucleus through interaction with KIP/CIP family members
Research Areas

BACKGROUND

Regulatory component of the cyclin D3-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D3/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex.

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