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Neurogenin3 Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 14 - Neurogenin3 Antibody (N-term) AP2024A
    AP2024A staining Neurogenin3 in human pancreas tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
  • 14 - Neurogenin3 Antibody (N-term) AP2024A
    AP2024A staining Neurogenin3 in human small intestine tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
  • 1 - Neurogenin3 Antibody (N-term) AP2024A
    All lanes : Anti-Neurogenin3 Antibody (N-term) at 1:2000 dilution Lane 1: DU145 whole cell lysate Lane 2: LNCap whole cell lysate Lane 3: PANC-1 whole cell lysate Lane 4: PC-3 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 23 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - Neurogenin3 Antibody (N-term) AP2024A
    The Neurogenin3 (N-term) polyclonal antibody (Cat. #AP2024a) is used in Western blot to detect Neurogenin3 in mouse liver tissue lysate.
  • 1 - Neurogenin3 Antibody (N-term) AP2024A
    Neurogenin3 Antibody (N-term) (Cat. #AP2024a) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the Neurogenin3 antibody detected the Neurogenin3 protein (arrow).
  • 3 - Neurogenin3 Antibody (N-term) AP2024A
    Confocal immunofluorescent analysis of Neurogenin3 Antibody (N-term) (Cat#AP2024a) with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IF, IHC-P, E
Primary Accession Q9Y4Z2
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Additional info
Gene ID 50674
Other Names Neurogenin-3, NGN-3, Class A basic helix-loop-helix protein 7, bHLHa7, Protein atonal homolog 5, NEUROG3, ATOH5, BHLHA7, NGN3
Target/Specificity This Neurogenin3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 40-69 amino acids from the N-terminal region of human Neurogenin3.
Dilution IHC-P~~1:25
WB~~1:1000
IF~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsNeurogenin3 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NEUROG3
Synonyms ATOH5, BHLHA7, NGN3
Function Acts as a transcriptional regulator. Together with NKX2- 2, initiates transcriptional activation of NEUROD1. Involved in neurogenesis. Also required for the specification of a common precursor of the 4 pancreatic endocrine cell types (By similarity).
Cellular Location Nucleus {ECO:0000255|PROSITE- ProRule:PRU00981}
Research Areas

BACKGROUND

Neurogenin-3 belongs to a family of basic helix-loop-helix transcription factors involved in the determination of neural precursor cells in the neuroectoderm. Neurogenin-3 also required for the specification of a common precursor of the 4 pancreatic endocrine cell types. Defects in NEUROG3 are the cause of congenital malabsorptive diarrhea 4 (DIAR4). DIAR4 is an autosomal recessive disorder characterized by generalized malabsorption and a paucity of enteroendocrine cells.

REFERENCES

Heremans, Y., et al., J. Cell Biol. 159(2):303-312 (2002). Sommer, L., et al., Mol. Cell. Neurosci. 8(4):221-241 (1996).

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