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NCAPH2 Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - NCAPH2 Antibody (N-term) AP1973A
    Western blot analysis of anti-NCAPH2 Pab (Cat. #AP1973a) in HepG2 cell line lysate (35ug/lane). NCAPH2(arrow) was detected using the purified Pab.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, E
Primary Accession Q6IBW4
Other Accession Q9BUT3
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 68227 Da
Additional info
Gene ID 29781
Other Names Condensin-2 complex subunit H2, Chromosome-associated protein H2, hCAP-H2, Kleisin-beta, Non-SMC condensin II complex subunit H2, NCAPH2, CAPH2
Target/Specificity This NCAPH2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 198-227 amino acids from the N-terminal region of human NCAPH2.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsNCAPH2 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NCAPH2
Synonyms CAPH2
Function Regulatory subunit of the condensin-2 complex, a complex that seems to provide chromosomes with an additional level of organization and rigidity and in establishing mitotic chromosome architecture. May play a role in lineage-specific role in T-cell development (By similarity).
Cellular Location Nucleus. Chromosome. Note=Distributed along the arms of chromosomes assembled in vivo and in vitro
Research Areas
Caspase-3-mediated degradation of condensin Cap-H regulates mitotic cell death.
Author : Lai SK, Wong CH, Lee YP, Li HY.
Cell Death Differ. 2011 Jun;18(6):996-1004. doi: 10.1038/cdd.2010.165. Epub 2010 Dec 10.
21151026
Detection of condensin I and II in maturing pig oocytes.
Author : Lisková L, Susor A, Pivonková K, Sasková A, Karabínová P, Kubelka M.
Reprod Fertil Dev. 2010;22(4):644-52. doi: 10.1071/RD09068.
20353724

BACKGROUND

Structural maintenance of chromosomes (SMC) and non-SMC condensin proteins associate into complexes that have been implicated in the process of chromosome condensation. A crucial prerequisite for accurate segregation of replicated sister chromatids is the condensation of the chromosomes into a manageable form prior to metaphase. The condensin I complex consists of two SMC subunits, SMC2 and SMC4, and three non-SMC subunits, CAP-H, CAP-G, and CAP-D2. An alternative complex, the condensin II complex, contains alternate non-SMC subunits, CAP-G2, CAP-H2, and CAP-D3. CAP-H2 is also known as Non-SMC condensin II complex, subunit H2 (NCAPH2) or kleisin beta isoform 2. The three non-SMC subunits in the condensing complexes form a regulatory subcomplex that is required to activate the SMC ATPases and to promote mitosis-specific chromatin binding of the holocomplex. The precise individual functions of each non-SMC protein in activation remain to be determined.

REFERENCES

Ono,T., et al. Cell 115 (1), 109-121 (2003).
Schleiffer,A., et al. Mol. Cell 11 (3), 571-575 (2003).
Loftus, B.J., et al., Genomics 60(3):295-308 (1999).

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