|Application ||WB, E|
|Calculated MW||67305 Da|
|Other Names||BRCA1-associated protein, 632-, BRAP2, Impedes mitogenic signal propagation, IMP, RING finger protein 52, Renal carcinoma antigen NY-REN-63, BRAP (HGNC:1099), RNF52|
|Target/Specificity||This BRAP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 66-95 amino acids from the N-terminal region of human BRAP.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||BRAP Antibody(N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Negatively regulates MAP kinase activation by limiting the formation of Raf/MEK complexes probably by inactivation of the KSR1 scaffold protein. Also acts as a Ras responsive E3 ubiquitin ligase that, on activation of Ras, is modified by auto- polyubiquitination resulting in the release of inhibition of Raf/MEK complex formation. May also act as a cytoplasmic retention protein with a role in regulating nuclear transport.|
|Tissue Location||Expressed in breast epithelial cell lines.|
Provided below are standard protocols that you may find useful for product applications.
The protein encoded by this gene was identified by its ability to bind to the nuclear localization signal of BRCA1 and other proteins. It is a cytoplasmic protein which may regulate nuclear targeting by retaining proteins with a nuclear localization signal in the cytoplasm.
Fulcher, A.J., et al. FASEB J. 24(5):1454-1466(2010)
Kamatani, Y., et al. Nat. Genet. 42(3):210-215(2010)
Ganesh, S.K., et al. Nat. Genet. 41(11):1191-1198(2009)
Hinohara, K., et al. J. Hum. Genet. 54(11):642-646(2009)
Cui, R., et al. Gastroenterology 137(5):1768-1775(2009)