|Application ||WB, E|
|Other Names||Endoribonuclease dcr-1, 3126-, dcr-1|
|Target/Specificity||This Dcr-1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1068-1097 amino acids from the Central region of human Dcr-1.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Dcr-1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Involved in cleaving double-stranded RNA in the RNA interference (RNAi) pathway (PubMed:11641272, PubMed:20223951). It produces 21 to 23 bp dsRNAs (siRNAs) which target the selective destruction of homologous RNAs (PubMed:11641272, PubMed:20223951). Seems to process the precursor of the small temporal RNA let-7 which is involved in developmental timing (PubMed:11641272).|
Provided below are standard protocols that you may find useful for product applications.
The Dcr-1 gene encodes a bidentate ribonuclease that is homologous to E. coli RNAse III. Dcr-1 is required both for RNA interference and for synthesis of small developmental RNAs. Dcr-1 interacts in vivo with RDE-4, a double-stranded RNA (dsRNA) binding protein required for RNAi that interacts with trigger dsRNAs and may function to deliver dsRNAs to Dcr-1 for endonucleolytic processing.
Duchaine TF, et al. 2006. Cell 124:343-354.
Denli AM, et al. 2004. Nature 432:231-235.
Tabara H, et al. 2002. Cell 109:861-871.
Grishok A, et al. 2001. Cell 106:23-34.
Knight SW, et al. 2001. Science 293:2269-2271.