|Application ||WB, IHC-P, E|
|Calculated MW||69151 Da|
|Other Names||Eukaryotic translation initiation factor 4B, eIF-4B, EIF4B|
|Target/Specificity||This EIF4B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 400-429 amino acids from human EIF4B.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||EIF4B Antibody (S422) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'- terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F.|
EIF4B is required for the binding of mRNA to ribosomes. It functions in close association with EIF4-F and EIF4-A by binding near the 5'- terminal cap of mRNA in presence of EIF-4F and ATP. It also promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F
Doepker, R.C., et al., J. Virol. 78(9):4684-4699 (2004).
Fleming, K., et al., Biochemistry 42(30):8966-8975 (2003).
Milburn, S.C., et al., EMBO J. 9(9):2783-2790 (1990).