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NEIL3 Antibody(Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - NEIL3 Antibody(Center) AP19397C
    All lanes : Anti-NEIL3 Antibody (Center) at 1:1000-1:2000 dilution Lane 1: MOLT-4 whole cell lysate Lane 2: A549 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 68 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, E
Primary Accession Q8TAT5
Other Accession NP_060718.2
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 67769 Da
Additional info
Gene ID 55247
Other Names Endonuclease 8-like 3, 322-, DNA glycosylase FPG2, DNA glycosylase/AP lyase Neil3, Endonuclease VIII-like 3, Nei-like protein 3, NEIL3
Target/Specificity This NEIL3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 292-320 amino acids from the Central region of human NEIL3.
Dilution WB~~1:1000-1:2000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsNEIL3 Antibody(Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NEIL3
Function DNA glycosylase which prefers single-stranded DNA (ssDNA), or partially ssDNA structures such as bubble and fork structures, to double-stranded DNA (dsDNA). In vitro, displays strong glycosylase activity towards the hydantoin lesions spiroiminodihydantoin (Sp) and guanidinohydantoin (Gh) in both ssDNA and dsDNA; also recognizes FapyA, FapyG, 5-OHU, 5-OHC, 5- OHMH, Tg and 8-oxoA lesions in ssDNA. No activity on 8-oxoG detected. Also shows weak DNA-(apurinic or apyrimidinic site) lyase activity. In vivo, appears to be the primary enzyme involved in removing Sp and Gh from ssDNA in neonatal tissues. Seems to be an important facilitator of cell proliferation in certain populations, for example neural stem/progenitor cells and tumor cells, suggesting a role in replication-associated DNA repair.
Cellular Location Nucleus.
Tissue Location Expressed in keratinocytes and embryonic fibroblasts (at protein level). Also detected in thymus, testis and fetal lung primary fibroblasts.
Research Areas

BACKGROUND

NEIL3 belongs to a class of DNA glycosylases homologous to the bacterial Fpg/Nei family. These glycosylases initiate the first step in base excision repair by cleaving bases damaged by reactive oxygen species and introducing a DNA strand break via the associated lyase reaction (Bandaru et al., 2002 [PubMed 12509226]).

REFERENCES

Krokeide, S.Z., et al. Protein Expr. Purif. 65(2):160-164(2009)
Takao, M., et al. Genes Cells 14(2):261-270(2009)
Dallosso, A.R., et al. Gut 57(9):1252-1255(2008)
Bethke, L., et al. J. Natl. Cancer Inst. 100(4):270-276(2008)
Newton-Cheh, C., et al. BMC Med. Genet. 8 SUPPL 1, S7 (2007) :

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