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>   首页   >   产品   >   一抗   >   免疫学   >   Mouse Trem2 Antibody (N-term)   

Mouse Trem2 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 59 - Mouse Trem2 Antibody (N-term) AP19275a
    Immunohistochemical analysis of paraffin-embedded mouse lung tissue using AP19275a performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
  • 59 - Mouse Trem2 Antibody (N-term) AP19275a
    Immunohistochemical analysis of paraffin-embedded mouse brain tissue using AP19275a performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
  • 1 - Mouse Trem2 Antibody (N-term) AP19275a
    All lanes : Anti-Mouse Trem2 Antibody (N-term) at 1:500 dilution Lane 1: Mouse lung tissue lysate Lane 2: Mouse liver tissue lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 25 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - Mouse Trem2 Antibody (N-term) AP19275a
    All lanes : Anti-Mouse Trem2 Antibody (N-term) at 1:500-1000 dilution Lane 1: Mouse kidney tissue lysate Lane 2: Mouse testis tissue lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 25 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - Mouse Trem2 Antibody (N-term) AP19275a
    Anti-Mouse Trem2 Antibody (N-term) at 1:2000 dilution + Mouse liver lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 25 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - Mouse Trem2 Antibody (N-term) AP19275a
    All lanes : Anti-Mouse Trem2 Antibody (N-term) at 1:2000 dilution Lane 1: mouse brain lysate Lane 2: mouse lung lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 25 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IHC-P-Leica, WB, E
Primary Accession Q99NH8
Other Accession NP_112544.1
Reactivity Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 24527 Da
Additional info
Gene ID 83433
Other Names Triggering receptor expressed on myeloid cells 2, TREM-2, Triggering receptor expressed on monocytes 2, Trem2, Trem2a, Trem2b, Trem2c
Target/Specificity This Mouse Trem2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 22-48 amino acids from the N-terminal region of mouse Trem2.
Dilution IHC-P-Leica~~1:500
WB~~1:2000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMouse Trem2 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name Trem2
Synonyms Trem2a, Trem2b, Trem2c
Function Forms a receptor signaling complex with TYROBP and triggers activation of the immune responses in macrophages and dendritic cells. May have a role in chronic inflammations and may stimulate production of constitutive rather than inflammatory chemokines and cytokines.
Cellular Location Isoform 1: Cell membrane {ECO:0000250|UniProtKB:Q9NZC2}; Single-pass type I membrane protein
Tissue Location Expressed at higher levels in the CNS, heart and lung than in lymph nodes or in other non-lymphoid tissues such as kidney, liver and testis. In the CNS not all microglia express TREM2. Brain regions with an incomplete blood-brain barrier had the lowest percentages of TREM2 expressing microglia, whereas the lateral entorhinal and cingulate cortex had the highest percentages.
Research Areas

BACKGROUND

Trem2 may have a role in chronic inflammations and may stimulate production of constitutive rather than inflammatory chemokines and cytokines. Forms a receptor signaling complex with TYROBP and triggers activation of the immune responses in macrophages and dendritic cells.

REFERENCES

Koth, L.L., et al. J. Immunol. 184(11):6522-6528(2010)
Whittaker, G.C., et al. J. Biol. Chem. 285(5):2976-2985(2010)
Peng, Q., et al. Sci Signal 3 (122), RA38 (2010) :
Chang, J.H., et al. Biochem. Biophys. Res. Commun. 389(1):28-33(2009)
Hsieh, C.L., et al. J. Neurochem. 109(4):1144-1156(2009)

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