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FOXN3 Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - FOXN3 Antibody (C-term) AP19255B
    All lanes : Anti-FOXN3 Antibody (C-term) at 1:2000 dilution Lane 1: 293T/17 whole cell lysate Lane 2: Jurkat whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 54 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 3 - FOXN3 Antibody (C-term) AP19255B
    Fluorescent image of Hela cell stained with FOXN3 Antibody (C-term)(Cat#AP19255b).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with FOXN3 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃).FOXN3 immunoreactivity is localized to Nucleus significantly.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IF, WB, E
Primary Accession O00409
Other Accession NP_005188.2
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Additional info
Gene ID 1112
Other Names Forkhead box protein N3, Checkpoint suppressor 1, FOXN3, C14orf116, CHES1
Target/Specificity This FOXN3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 380-408 amino acids from the C-terminal region of human FOXN3.
Dilution WB~~1:2000
IF~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsFOXN3 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name FOXN3
Synonyms C14orf116, CHES1
Function Acts as a transcriptional repressor. May be involved in DNA damage-inducible cell cycle arrests (checkpoints).
Cellular Location Nucleus.
Research Areas
Bispecific Forkhead Transcription Factor FoxN3 Recognizes Two Distinct Motifs with Different DNA Shapes.
Author : Rogers JM1,Waters CT2,Seegar TCM3,Jarrett SM4,Hallworth AN5,Blacklow SC6,Bulyk ML7.
Mol Cell. 2019 Feb 22. pii: S1097-2765(19)30039-5. doi: 10.1016/j.molcel.2019.01.019. [Epub ahead of print]
30826165
A Hepatocyte FOXN3-α Cell Glucagon Axis Regulates Fasting Glucose.
Author : Karanth S1,Adams JD2,Serrano MLA3,Quittner-Strom EB4,Simcox J5,Villanueva CJ5,Ozcan L6,Holland WL7,Yost HJ8,Vella A2,Schlegel A9.
Cell Rep. 2018 Jul 10;24(2):312-319. doi: 10.1016/j.celrep.2018.06.039.
29996093
Checkpoint suppressor 1 suppresses transcriptional activity of ERα and breast cancer cell proliferation via deacetylase SIRT1.
Author : Xu Z1,Yang Y1,Li B1,Li Y1,Xia K1,Yang Y1,Li X1,Wang M1,Li S1,Wu H2.
Cell Death Dis. 2018 May 11;9(5):559. doi: 10.1038/s41419-018-0629-3.
29752474
FOXN3 Regulates Hepatic Glucose Utilization.
Author : Karanth S1, Zinkhan EK2, Hill JT3, Yost HJ4, Schlegel A5.
Cell Rep. 2016 Jun 8. pii: S2211-1247(16)30654-4. doi: 10.1016/j.celrep.2016.05.056. [Epub ahead of print]
27292639

BACKGROUND

This gene is a member of the forkhead/winged helix transcription factor family. Checkpoints are eukaryotic DNA damage-inducible cell cycle arrests at G1 and G2. Checkpoint suppressor 1 suppresses multiple yeast checkpoint mutations including mec1, rad9, rad53 and dun1 by activating a MEC1-independent checkpoint pathway. Alternative splicing is observed at the locus, resulting in distinct isoforms. [provided by RefSeq].

REFERENCES

Samaan, G., et al. Biochem. Biophys. Res. Commun. 400(1):60-65(2010)
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Jugessur, A., et al. PLoS ONE 5 (7), E11493 (2010) :
Markowski, J., et al. Eur Arch Otorhinolaryngol 266(10):1501-1507(2009)
Schlade-Bartusiak, K., et al. Am. J. Med. Genet. A 146A (1), 117-123 (2008) :

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